正交试验法优选延胡索中延胡索乙素提取工艺

Extraction Technology Optimization for Tetrahydropalmatine from Corydalis Yanhusuo by Orthogonal Experiment

目的:优选延胡索中延胡索乙素的最佳提取工艺。方法:选用L_9(3~4)正交试验设计,以提取物中延胡索乙素的含量为指标,考察乙醇浓度、乙醇用量、提取温度和提取时间对提取效果的影响。结果:最佳提取工艺为按料液比1∶10(g·ml^(-1))加入浓度为60%的乙醇溶液,以80°C为提取温度,加热回流提取3次,每次1 h。结论:优选得到提取工艺稳定、合理、可行、操作简单,能耗低,较大限度地提高了延胡索的综合利用率。

Objective： To optimize the extraction technology of tetrahydropalmatine in Corydalis yanhusuo. Methods： An L9 （34） orthogonal experiment was performed to investigate the effects of ethanol concentration, ethanol amount, extraction temperature and ex- traction time on the extraction results with the content of tetrahydropalmatine as the evaluation index. Results ： The optimum conditions were as follows： the solid-liquid ratio was 1 ： 10 （g ml-l）, 60% ethanol was used as the extraction solvent, the extraction tempera- ture was at 80 ℃ for three-time extraction, and the extraction duration for each time was 1 h. Conclusion： The optimized extraction technology for tetrahydropalmatine from Corydalis yanhusuo is stable, reasonable, feasible and easy-operated with low energy consump- tion, which can enhance the comprehensive utilization of Corydalis yanhusuo.