麝香保心丸中5种人参皂苷类化合物在心肌梗死大鼠血浆中的药代动力学研究

Pharmacokinetics of five ginsenosides of Shexiang Baoxin pill in plasma of myocardial infarction rats

麝香保心丸(SBP)是治疗冠心病的经典复方,其中人参皂苷类化合物是其发挥药效的主要成分之一。该文采用液相色谱串联质谱(LC-MS/MS)的方法,研究心梗大鼠多次灌胃麝香保心丸、人参提取物(GE)及5种人参皂苷类单体化合物后人参皂苷类化合物的药代动力学特性。研究发现人参皂苷类化合物总体吸收较差,各组Cmax均小于200μg·L-1。比较各组分析物的Tmax发现人参提取物组和麝香保心丸组中人参皂苷类化合物均比各单一化合物组吸收快,提示人参提取物及复方中均存在促进人参皂苷成分吸收的成分。麝香保心丸组中的各人参皂苷成分的给药剂量在所有给药组中最小,但相应的AUC0-t和AUCINF数值最大,表明配伍后麝香保心丸中人参皂苷类成分吸收最好,说明复方是中医药用的最佳形式。

Shexiang Baoxin pill(SBP) is widely used for treating coronary heart disease in clinic,with ginsenosides as its main effective component. This study was designed to investigate and compare the pharmacokinetic characteristics of five ginsenosides of five compounds after multiple oral administrations,ginseng extract(GE) and SBP in myocardial infarction rats. After intragastric administration to myocardial infarction rats,the plasma samples were analyzed by liquid chromatography tandem triple-quad mass spectrometry. The results showed that Cmaxof five compounds in all groups were less than 200 μg·L-1. Tmaxof corresponding analytes between groups revealed that ginsenosides in both SBP and GE were absorbed faster than each of the five compounds,indicating that GE and compounds contain components promoting absorption of ginsenosides. The oral administration doses of ginsenosides in SBP were the least in all groups,but with the greatest AUC0-tand AUCINF,which indicated that ginsenosides in SBP had the best absorption in all groups after oral administration to myocardial infarction rats. This study also demonstrated that compound is the best form of traditional Chinese medicine.