Tim-3在恶性黑色素瘤患者外周血CD8^+T细胞上的表达及临床意义

Expression and clinical significance of Tim-3 on CD8^+T cell from peripheral blood of melanoma patients

目的:本研究应用流式细胞术检测恶性黑色素瘤患者外周血CD8^+T细胞中T细胞免疫球蛋白及黏蛋白-3(Tim-3)的表达情况,探讨Tim-3在恶性黑色素瘤进展中的作用。方法:应用流式细胞仪检测42例恶性黑色素瘤患者及38例健康体检者外周血CD8^+T细胞上Tim-3的表达,同时分析其与恶性黑色素瘤患者临床病理特征的关系。结果:Tim-3在恶性黑色素瘤患者及健康体检者外周血CD8+T细胞中均有不同程度的表达,恶性黑色素瘤组Tim-3表达量为(15.89±6.49)%,健康对照组的表达量为(3.27±2.16)%,差异具有统计学意义(P<0.01)。Tim-3在CD8^+T细胞中的表达水平与恶性黑色素瘤患者有无溃疡、淋巴结转移、临床分期、远处转移有关(P<0.01),而与性别、年龄、Breslow分度无关(P>0.05)。结论:Tim-3在恶性黑色素瘤患者外周血CD8^+T细胞上高水平表达,其表达水平与临床分期呈正相关,并有望成为恶性黑色素瘤早期预测及治疗的重要靶标。

Objective： To explore the role of T cell immunoglobulin and mucin domain molecules-3（ Tim-3） in malignant melanoma progression via detecting Tim-3 expression on CD8＋T Cells from peripheral blood of malignant melanoma patients. Methods： Detect Tim-3 expression on CD8~＋T cells from peripheral blood of 42 malignant melanoma patients and 38 healthy volunteers using flow cytometry,and analyze its relationship with clinical pathological features of the malignant melanoma patients. Results： The expression of Tim-3 CD8~＋T cell existed in both malignant melanoma patients and healthy volunteers,however,the proportion of Tim-3 CD8~＋T cell in malignant melanoma group（ 15. 89 ± 6. 49） % was significantly higher than the control group（ 3. 27 ± 2. 16） %,P〈0. 01. Higher expression of Tim-3 CD8~＋T cells in peripheral blood mononuclear cell was related to ulceration,lymph node metastasis,TNM stage,and distant metastasis in malignant melanoma patients（ P〈0. 01）,but not related to gender and age（ P〈0. 05）. Conclusion： Malignant melanoma patient＇s peripheral blood CD8~＋T cell expressed higher levels of Tim-3 compared to healthy donors,moreover,the expression level had positive correlation with TNM stage. Tim-3 may be a valuable molecular marker and an important therapeutic target.