一种比率型Cu^2＋电化学传感器及其分析应用

A Ratiometric Electrochemical Biosensor for Determination of Cu^(2+) and Its Bioanalytical Application

本研究以硫堇聚合物作为内参比探针分子,通过电化学聚合法将硫堇聚合于单壁碳纳米管修饰玻碳电极(SWNTs/GCE)上,并以该修饰电极为工作电极,建立了一种比率型检测Cu^(2+)的电化学传感方法。结果表明,在1~25μmol/L浓度范围内,Cu^(2+)的阳极溶出峰电流ICu^(2+)与聚硫堇氧化峰电流ITh的比值与Cu^(2+)浓度呈较好的线性关系,检测限为96nmol/L,方法用于加标自来水样中Cu^(2+)的检测,回收率较好。

Thionine polymer（polythionine,poly-Th）was used as an internal reference probe,and was coated on a single-walled carbon nanotubes（SWNTs） modified glassy carbon electrode by electropolymerization method.As-fabricated modified electrode was used as the working electrode by anodic stripping voltammetry for the detection of copper ions,and a ratiometric electrochemical method was established for the determination of Cu^2＋.The results indicated that under the optimum conditions in the Cu^2＋concentration range 1-25μmol/L,the ratio of the anodic stripping peak current of Cu^2＋（ICu^2＋）and the oxidation peak current of poly-Th（ITh）was proportional to the concentration of Cu^2＋with the detection limit of 96nmol/L.The method can be applied to the determination of Cu^2＋in spiked tap water samples with good recovery,which establishes the foundation for fast,simple,accurate determination of Cu^2＋using ratiometric electrochemical method.