硫氧还原蛋白过氧化物酶1在肝癌组织中表达的临床意义

Expression of peroxiredoxin 1 in hepatocellular carcinoma and its clinical significance

目的探讨硫氧还原蛋白过氧化物酶1（peroxiredoxin 1，Prx1）在肝癌组织及癌旁组织中表达的意义。方法随机选取人肝癌组织及癌旁组织标本80例，石蜡包埋切片后采用免疫组化SP方法染色及RT-PCR法观察Prx1蛋白和mRNA在癌组织及癌旁组织中的表达水平，分析Prx1蛋白差异性表达的意义及与临床病理的关系。结果Prx1主要在肝癌组织及癌旁组织胞质中表达。Prx1在肝癌组织中的蛋白和mRNA表达水平明显高于癌旁组织（P〈0．05）。Prx1的表达与有无血管侵犯（P〈0．01）和TNM分级有关（P〈0．05），而与患者年龄、性别、肿瘤大小、肿瘤数目、包膜是否完整及甲胎蛋白（AFP）水平无关（P〉0．05）。结论Prx1在肝癌组织及癌旁组织中表达水平上调，参与了肝癌的发生、发展。

Objective To investigate the levels of peroxiredoxin 1 （ Prx 1 ） in hepatocellular carcinoma （HCC） and adjacent tissue and its clinical significance. Methods A total of 80 samples of human HCC and adjacent tissues were randomly selected for paraffin embedding and sectioning. The resultant slices were used for immunohistochemical staining SP and RT - PCR to observe the levels of Prx 1 protein and mRNA in HCC and adjacent tissues and analyze the relation- ship between the differential expression of Prx 1 protein and clinical pathological charateristics. Results Prx 1 was mainly expressed in the cytoplasm of HCC and adjacent tissues. The levels of Prx 1 protein and mRNA were remarkably higher in HCC tissues than those in the adjacent tissues （ P 〈 0.01 ）. The expression of Prx 1 was associated with vascu- lar invasion （ P 〈 0.01 ） and TNM classification （ P 〈 0.05 ）, rather than a patient＇s age, gender, tumor size and num- ber, intact envelop and alpha fetal protein （AFP） level （ P 〉 0.05 ）. Conclusions The expression of Prx I is up - reg- ulated in HCC and adjacent tissues, and Prx 1is involved in pathogenesis of hepatic cancer.