摘要
采用Fmoc固相合成策略,以Wang树脂为载体,Fmoc保护的氨基酸为原料,HOBT/HBTU/DIEA为缩合剂,经RP-HPLC分离纯化合成了3条多肽(BRC4,BRC4-1和BRC4-2),纯度>90%,其结构经MS(ESI)确证。采用圆二色光谱研究了BRC4,BRC4-1和BRC4-2与蛋白RAD51关键肽段RAD51(231-260)的相互作用。结果表明:3条多肽与RAD51(231-260)的相互作用强度为BRC4-1>BRC4-2>BRC4。
Three polypeptides（ BRC4, BRC4-1 and BRC4-2）, with over 90% purity, were synthe- sized by the Fmoc solid synthesis strategy then separation and purification by RP-HPLC, using Wang resin as carrier, Fmoc protected amino acid as material and HOBT/HBTU/DIEA as coupling reagents. The structures were confirmed by MS （ESI）. The interactions of BRC4, BRC4-1 and BRC4-2 with RAD51 were investigated by circular dichroism. The circular dichroism results showed that the sequence of interaction strength with RAD51 （236-260） was BRC4-1 〉 BRC4-2 〉 BRC-4.
作者
马丽
纪浩杰
王莹
赵东欣
卢奎
MA Li JI Hao-jie WANG Ying ZHAO Dong-xin LU Kui(School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450052, China School of Materials and Engineering, Henan Institute of Engineering, Zhengzhou 451191, China)
出处
《合成化学》
CAS
CSCD
2017年第2期137-139,143,共4页
Chinese Journal of Synthetic Chemistry
基金
国家自然科学基金资助项目(21172054
21572046)
河南省教育厅自然科学基金资助项目(14A150017)
