高频电疗对大鼠脑缺血再灌注损伤后caspase-3和海马神经细胞超微结构的影响

Effect of high frequency electrotherapy on caspase-3 and ultra microstructure of hippocampus in rats following cerebral ischemia/reperfusion

目的:观察高频电疗对大鼠脑缺血再灌注损伤后caspase-3和海马神经细胞超微结构的影响。方法:线栓法制备大鼠一侧大脑中动脉缺血再灌注(middle cerebral artery occlusion and reperfusion,MCAO/R)模型。采用Zea-Longa评分法评定神经功能缺损程度。将符合条件的大鼠随机分成假手术组、模型组及高频电疗组。1,3,7 d后取标本,电镜观察海马神经元细胞超微结构,Western印迹检测caspase-3蛋白的表达。结果:电镜示假手术组海马CA1区神经细胞完整,各细胞器结构、形态正常。模型组海马神经元肿胀、坏死,细胞器碎裂,神经细胞损伤严重。高频电疗后海马神经元损伤均较模型组减轻。同时间点(3,7 d)高频电疗组caspase-3升高程度较模型组降低(P<0.05)。结论:高频电疗可改善脑缺血再灌注损伤大鼠的神经功能,抑制caspase-3及脑神经细胞凋亡,减轻神经元超微结构损伤,有一定的脑保护作用。

Objective： To investigate the effect of high after cerebral ischemia/reperfusion （I/R）. frequency electrotherapy （HFE） on rat hippocampus Methods： A rat model of cerebral I/R injury was established. The rats were randomly divided into a sham group, an I/R group and an HFE group. The HFE group received thearapy daily for different sessions for 1, 3, 7 d. Neuronal deficit score, neuron ultra microstructure in the hippocampus and caspase-3 protein expression were measured on 1 st, 3 th and 7th d.Results： Compared with the I/R group, the HFE group showed the decreased neurological deficit scores, with significant differences between the 2 groups （P〈0.05）. Tne injury in HFE group was reduced compared with that in the I/R group based on the electron microscope test, with significant difference. Caspase-3 protein in brain tissue in the HFE group also downregulated compared with that in the I/R group （P〈0.05）. Conclusion： High frequency electrotherapy can improve neural function, suppress caspase-3 expression and apoptosis in nerve cells and improve the ultra microstructure of neurons, displaying a protective effect on cerebral I/R injury in rats.