橡胶树红根病菌Fip-gpo基因的克隆及启动子分析

Cloning and Promoter Sequence Analysis of Fip-gpo Gene from Ganoderma pseudoferreum

为预测橡胶树红根病菌(Ganoderma pseudoferreum)真菌免疫调节蛋白基因的序列特点和表达模式,采用同源克隆获得Fipgpo的c DNA和DNA的全长序列。结果表明:该基因编码区全长934 bp,编码111个氨基酸残基,分子量为12.54 k D,等电点4.66,有4个ATM磷酸化位点,与赤灵芝(G.lucidum)的Fip序列相似性为91%;其启动子区域含有59 bp的内含子,除了含有TATA-box、CAAT-box基本元件外,还含有参与光应答/调控元件、赤霉素响应元件、茉莉酸甲酯应答元件、厌氧诱导作用元件、调控在胚乳中特异表达的顺式作用元件、分生组织特异激活顺式元件及生长素反应元件。推测Fip-gpo基因的表达受激素、非生物诱导、光照等的调控。

This experiment was conducted to investigate the sequence characteristics and expression pattern of fungal immunomodulatory protein gene in Ganoderma pseudoferreum. The eDNA, DNA and promoter sequences of a Fip-gpo gene by homologous cloning from G. Pseudoferreum were isolated and analyzed. The result showed that the fragment contained a full encoding region of 934 bp encoding 111 amino acid residues with a molecular mass of 12.54 kD, this deduced protein had a pI of 4.66, four ATM Phosphorylation sites prediction, and similarity with Fip sequence of G. lucidum in 91% ; Its DNA promoter sequence had 59 bp intron, in addition to the TATA/CAATbox, its promoter contains some specific regulatory elements such as light responsive element and cis-acting regulatory element involved in light responsiveness, gibbereUin-responsive element, cis-actiug regulatory element involved in the MeJA-responsiveness, cis-acting regulatory element essential for the anaerobic induction, cis-acting regulatory element required for endosperm expression, cis-aeting regulatory element related to meristem specific activation and anxin-responsive element. It was supposed that the expression of Fip-gpo gene was regulated by circadian, abiotic elicitor, light et al.