基于香蕉叶片解剖结构的抗寒性评价

Cold Resistance Evaluation Based on Leaf Anatomy Structure of Banana

利用石蜡切片技术对不同抗寒性的香蕉叶片解剖结构进行研究,为香蕉的抗寒育种提供参考。结果表明,香蕉叶片属典型的异面叶,具有复表皮结构。海绵组织厚度在不同香蕉种质间的变化最大,其次为栅海比。通过聚类分析和相关性分析筛选出海绵组织厚度和栅海比作为香蕉种质抗寒性评价的解剖结构指标。通过隶属函数对18个香蕉种质进行了综合评价,其抗寒性大小顺序为:LT>粉杂>金粉1号>广东粉蕉>HA>LE>LNF>SNY>SNT>NLF>HR>桂蕉青7号>HLT>LNS>SDW>桂蕉6号>ZNF>HLE。通过聚类分析,可以将这些种质分为3类,其中LT、粉杂和金粉1号在供试种质中抗寒性最强。

The Leaf transection structure of different cold resistance banana have been studied by using paraffin section technology to offer ref- erences for breeding of cold resistance of banana. The result showed the banana leaf belonged to typical bifacial leaf with a compound epidermis. Thickness of spongy tissue was the most variable in different bananas, followed by palisade tissue and spongy tissue ratio. Leaf tissue structure indexes were screened by correlation and hierarchical cluster analysis, spongy tissue thickness and palisade tissue and spongy tissue ratio were selected as the anatomy structure indexes to evaluate the cold resistance of banana. Then the cold resistance evaluation of 18 bananas was done by subordinate function, they declined in sequence from LT, Fenza, Jinfen 1, Guangdongfenjiao, HA, LE, LNF, SNY, SNT, NLF, HR, Guijiaoqing 7, HLT, LNS, SDW, Guijiao 6, ZNF, to HLE. Also, these germplasms could be classified into three groups by hierarchical cluster analysis. The cold-resistance of LT, Fenza and Jinfen lwere higher than others.