蓝莓间座壳茎溃疡病病原鉴定及生物学特性研究

Identification and biological characteristics of a blueberry Diaporthe stem canker pathogen

在蓝莓园进行病样采集时,发现一种蓝莓茎部病害,病斑呈红褐色,溃疡状,长椭圆形。为明确该病害的病原菌种类,对病枝进行组织分离,经单孢纯化后得到菌落形态一致的7株致病菌,对供试菌株的ITS与EF1-α基因联合构建系统发育树,供试菌株与Diaporthe phaseolorum处于同一分支,结合形态学特征,鉴定该病原菌为Diaporthe phaseolorum。对病原菌的生物学特性研究结果表明,该菌最适培养基为PDA,最适生长温度为25℃,光照对菌丝生长无明显影响。本文首次报道Diaporthe phaseolorum引起蓝莓茎溃疡病。

A kind of blueberry stem canker was found in blueberry plantation and the symptoms consist of red- brown and elliptical stem canker. In order to identify the pathogen, seven isolates were obtained by isolation and purification. Phylogenetic analysis of combined ITS and EFI-α gene sequences showed that the isolates were loca- ted in the same clade with Diaporthe phaseolorum. Compared with the morphological characteristics, the isolates were identified as D. phaseoIorum. The biological characteristics test showed that 25℃, PDA were the best cul- ture conditions, and it was not sensitive to light. This is the first report of D. phaseolorum causing diseases on blueberry.