山东烟台地区发生番茄斑萎病毒病危害

Identification of Tomato spotted wilt virus disease in Shandong,China

番茄斑萎病毒Tomato spotted wilt virus(TSWV)是我国进境植物检疫性有害生物,近年来相继在国内一些省份发现。利用TSWV的通用引物NF302/NR575对从山东烟台地区收集的15份疑似感染番茄斑萎病毒病的番茄样品进行检测;进一步对特异引物TSWV-NF2037/TSWV-NR2825扩增的TSWV的N基因序列克隆、测序,并对N基因片段编码氨基酸进行遗传距离及系统发育分析。结果表明,15份疑似样品中有4个样品扩增得到TSWV病毒片段;基于N基因序列分析发现,山东TSWV番茄分离物与云南TSWV番茄分离物(AEI70836.1)的遗传距离最近,为0.8%,且与云南TSWV番茄分离物聚为一支。这是山东地区首次利用分子标记证实番茄斑萎病毒病的危害。

Tomato spotted wilt virus （TSWV） is an important plant quarantine pest, which has been foand in some prov- inces of China in recent years. In this study, 15 suspected tomato samples collected from Yantai, Shandong were first de- tected by using PCR with the TSWV-universal primers NF302/NR575. Then, the N gene of TSWV isolates amplified by specific primers TSWV-NF2037/TSWV-NR2825 was sequenced for phylogenetic analysis and genetic distance analysis. The results based on the detection with NF302/NR575 showed that four of the 15 samples were positive for TSWV. Ge- netic distance analysis based on the N gene sequences showed that the genetic distance between Shandong isolate and Yun- nan （AEI70836.1） was the closest （i. e. 0.8%）. Phylogenetic analysis showed that the Shandong isolate was closely re- lated to the Yunnan isolate （AEI70836.1）. Our results proved the introduction of TSWV into Shandong, which is the first evidence of TSWV disease in Shandong based on molecular markers.