超高效液相质谱联用法测定阿昔洛韦血药浓度

Determination of Acyclovir in Human Plasma by UPLC-MS/MS

目的建立UPLC-MS/MS法测定人血浆中阿昔洛韦浓度,为其药动学研究提供测定方法。方法色谱条件:Acquity UPLC BEHC18色谱柱(2.1mm×50mm,1.7μm)、流动相为甲醇-0.01%甲酸水(60︰40,V/V)、流速0.20m L·min^(-1)。质谱条件:电喷雾离子源(ESI-)、多反应离子监测模式(MRM)、阿昔洛韦检测离子对为(m/z)255.0→152.0、毛细管电压3.7KV、锥孔电压30V、碰撞能量20V。血浆样品采用液-固取预处理后进样。结果血浆中阿昔洛韦线性范围50~1600 ng·m L^(-1);方法回收率为99.6%±6.4,日内、日间的RSD值均<10%,最低检测限为1ng·m L^(-1)(S/N≥3)。结论 UPLC-MS/MS法测定血浆中阿昔洛韦浓度灵敏度高、重现性好,且操作简便、试剂价廉低毒,适用于临床常规监测及药动学研究。

OBJECTIVE To establish determination method of acyclovir concentration inplasma by UPLC-MS /MS for its pharmacokinetic research. METHODS Chromatographic conditions： Acquity UPLC BEHC18（ 2. 1mm ×50mm,1. 7μm） Column,mobile phase was methanol-0. 01% formic acid（ 60 ︰ 40,V / V）,Flow rate was 0. 2m L·min^（-1）. MS conditions： ionsource electrospray ionization（ ESI-）,multiple reaction monitoring（ MRM）,detected ion pairs（ m / z） 255. 0→152. 0,capillary voltage 3. 7KV,cone voltage 30 V,the collision energy of 20 V. Plasma samples were pre-processed by liquid-solid extraction before sample injection. RESULTS The linear calibration curves of Acyclovir was obtained,and its concentration Linear relationship was in 50 ~ 1600 ng·m L^（-1）. Its method recoveries was 99. 6% ± 6. 4. The limit of detection was 1ng·m L^（-1）（ S /N≥3）. CONCLUSION The established UPLC-MS /MS method for determination is sensitive,Reproducibility,simple,inexpensive and low toxicity,which can be used for drug monitoring and pharmacokinetics study in clinical routine treatment.