外周血CD5^+B淋巴细胞与不明原因自然流产的关系性研究

Peripheral blood CD5^+B lymphocytes and spontaneous abortion of unknown origin

目的:通过检测不明原因自然流产患者和正常育龄妇女外周血CD5^+B淋巴细胞的含量,探讨CD5^+B淋巴细胞与不明原因自然流产的关系。方法:选择自然流产患者5例,正常育龄妇女5例。运用免疫磁珠法分离外周血中B淋巴细胞,采用流式细胞术检测CD5^+B淋巴细胞的含量。结果:正常育龄组外周血中CD5^+B淋巴细胞的比例为(0.78±0.40)%;自然流产组为(6.64±4.34)%。自然流产组明显高于正常育龄组,差异有统计学意义(P<0.05)。结论:CD5^+B淋巴细胞可能参与不明原因自然流产的发病过程。

Objective： To investigate the correlation of spontaneous abortion of unknown causes and CD5＋B lymphocytes through determination of the CD5 positive B lymphocyte level in the peripheral blood in women with unestablished miscarriage and normal pregnancy.Methods： Five patients with spontaneous abortion were included in the experimental group,and five women of normal pregnancy were recruited as control group.Magnetic activated cell sorting technique was used to（ MACS） to isolate the peripheral blood CD5＋B lymphocytes for which the level was measured with flow cytometry（ FCM）.Results：Women of normal pregnancy had higher level of peripheral blood CD5＋B lymphocytes than the patients with miscarriage [（ 6. 64 ± 4. 34） % vs.（ 0. 78 ±0.40） %].The difference was significant（ P〈0.05）.Conclusion： CD5＋B lymphocytes might be involved in the pathogenesis of the spontaneous abortion of unknown origin.