摘要
为了探讨miR-202在鱼类胚胎发育中的功能,采用实时定量反转录PCR技术和整体原位杂交技术检测了miR-202在斑马鱼胚胎发育阶段的表达。结果发现miR-202是母源性分子并在斑马鱼胚胎发育过程中持续表达,尤其在早期胚胎发育阶段表达水平较高。在此基础上,采用基因沉默技术在斑马鱼受精卵中显微注射miR-202的反义锁核苷酸,实时荧光定量反转录PCR技术和整体原位杂交技术结果显示miR-202反义锁核苷酸可以显著下调斑马鱼胚胎中miR-202表达水平,同时发现反义抑制miR-202后胚胎发育停滞在4 hpf时左右。共同注射miR-202前体可以部分挽救反义抑制miR-202后导致的胚胎发育停滞。本研究证明miR-202在斑马鱼胚胎发育过程中起着重要的调控作用,其功能是斑马鱼胚胎早期发育所必需的。为进一步探索miR-202在鱼类胚胎发育过程的功能奠定了基础。
To learn the role of miR-202 in the zebrafish embryonic development, miR-202 expression was detected using qRT-PCR and WISH. The results indicated that miR-202 is a maternal molecule and expressed continuously during the zebrafish embryonic development, especially at the early stages. Knockdown of miR- 202 was performed by microinjecting the locked nucleic acid modified antisense oligo of miR-202. The results showed that antisense oligo of miR-202 decreased miR-202 level significantly, accompanied with an early termination of the zebrafish embryonic development at around 4 hpf. And miR-202 inhibition induced early termination could be partly rescued by co-injection of pre-miR-202, indicating that miR-202 is required for the early zebrafish embryonic development. The present study for the first time unveils the essential role of miR-202 for the zebrafish embryonic development.
作者
许彦娜
韩兵社
郑英慧
戴中华
陈良标
XU Yanna HAN Bingshe ZHENG Yinghui DAI Zhonghua CHEN Liangbiao(College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai 201306, China Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2017年第1期1-7,共7页
Journal of Shanghai Ocean University
基金
国家自然科学基金(31130049)
上海市自然科学基金(13ZR1419500)
上海市教委水产学一流学科项目(沪教科2012-62)
