不同地理种群白纹伊蚊感染Wolbachia的检测和系统发育分析

Detection and phylogenetic analysis of Wolbachia in Aedes albopictus from different geographical populations

目的探讨我国不同地理种群白纹伊蚊感染沃尔巴克氏体(Wolbachina)的情况和多样性,为蚊媒病的生物防控提供基础资料。方法选用Wolbachia的表面蛋白基因(wsp),经PCR扩增测序后与GenBank下载的序列进行比较分析,以确定白纹伊蚊感染Wolbachia的情况和类型。结果 13个地理种群中有11个种群的样品均检测到Wolbachia感染,通过核苷酸同源性比较和分析证实我国白纹伊蚊感染的类型包括A群的wAlbA亚群,B群的wPip和wAlbB两个亚群,其中山东泰安和广西玉林种群的白纹伊蚊为A、B群共感染。系统发育分析显示wsp基因序列聚成两大支,即A群和B群;B群又分为wPip和wAlbB两支。结论我国不同地理种群的白纹伊蚊体内普遍感染Wolbachia,其基因型主要为wAlbA和wAlbB型。

Objective Investigate Wolbachia infection and diversity in Aedes albopictus from different populations of China, in order to provide baseline data for the prevention and control of mosquito-borne diseases. Methods Choosing Wolbachia surface protein gene （wsp） as reference. All wsp DNA sequences obtained through PCR and sequenced were used for comparison with those sequences downloaded from GenBank, to determine the states and types of Wolbachia that infected by Ae. albopictus. Results Eleven populations were infected with Wolbachia, which including wAlbA subgroup of A group, and wPip, wAlbB subgroup of B group. Additionally, samples from Taian and Yulin were found coinfected with A and B group. These results were further supported by phylogenetic analysis, which demonstrated that all wsp sequences were clustered into two clades, and two subclades in B group were corresponding with wPip and wAlbB. Conclusion Aedes albopictus of our country were mainly infected with wAlbA and wAlbB subgroups, which with high infect rate.