超声波辅助乙醇提取山葡萄籽多酚的工艺研究

Study on extracting polyphenols from Vitis amurensis seeds through ultrasound-assisted method using ethanol

目的:确定超声波辅助乙醇提取山葡萄籽多酚的最佳工艺条件,为山葡萄籽的利用提供技术参考。方法:以山葡萄籽为原料,乙醇为提取液,通过单因素实验与正交试验设计,检测在不同乙醇浓度(20%、40%、60%、80%和95%)、提取时间(10、20、30、40和60 min)、提取温度(25℃、35℃、45℃、55℃和65℃)、料液比(1∶4、1∶6、1∶8、1∶10和1∶12)、提取次数(1、2、3、4和5次)及超声功率(50、60、80、100和120 W)时山葡萄籽多酚的提取率。结果:超声波辅助乙醇提取山葡萄籽多酚的最佳工艺参数为乙醇浓度60%、提取温度50℃、提取时间30min、超声功率120 W、料液比1∶8,提取2次。在此工艺参数下,山葡萄籽多酚得率为11.71%,多酚含量为32.86%。结论:与传统溶剂浸提法比较,超声波辅助乙醇提取山葡萄籽多酚更具优势,该工艺具有简单、合理和高效的特点。

Objective： To establish the optimized ultrasound-assisted extraction process of polyphenols from Vitis amurensis seeds, and to provide technical reference for the use of grape seeds.Methods： With vitis amurensis seeds as raw material and ethanol as solvent, the extraction rates of polyphenols were detected under different conditions,such as different ethanol concentrations（20%,40%,60%,80%, and 95%）, extraction time（10,20,30,40,and 60 min）, extraction temperatures（25℃,35℃,45℃,55℃,and 65℃）, material/liguid ratios（1：4,1：8,1：10,and 1：12）,extraction times（1,2,3,4,and 5） and ultrasonic powers （50,60,80,100, and 120 w） by single factor experiment and orthogonal test.Results： The optimum parameters identified were as follows：ethanol concentration was 60%,extracting temperature was 50℃,extraction time was 30 min,ultrasonic power was 120 W,material/liquid ratio was 1：8, and extracting times was 2. Under the optimal extraction conditions, the extraction rate of polyphenols from Vitis amurensis seeds was 11.71%,and the polyphenols concentration was 32.86%.Conclusion： Compared with traditional solvent extraction, ultrasound-assisted extraction of polyphenols from Vitis amurensis seeds has more advantages; the craft is simple, reasonable and efficient.