Effects of astaxanthin on oxidative stress induced by Cu^(2+) in prostate cells

虾青素对铜离子诱导的前列腺细胞氧化损伤的影响（英文）

Astaxanthin（AST）, a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT） assay; cell apoptosis and intracellular reactive oxygen species（ROS） levels were determined by flow cytometry; the mitochondrial membrane potential（MMP） was measured by fluorospectrophotometer; and activities of malondialdehyde（MDA）, superoxide dismutase（SOD）, catalase（CAT）, and glutathione peroxidase（GSH-Px） were evaluated by a detection kit. The results show that copper ion（Cu^2＋） induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines（RWPE-1 and PC-3）. AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu^2＋, but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu^2＋-induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells.

目的:研究虾青素对铜离子诱导的前列腺细胞氧化损伤的影响,并探索其作用机制。创新点:首次研究虾青素对铜离子诱导的前列腺细胞及前列腺癌细胞氧化损伤的影响,并比较其对两种细胞作用的差异。方法:MTT法测定铜离子与虾青素对前列腺细胞(RWPE-1)和前列腺癌细胞(PC-3)生长的影响;采用细胞流式仪测定虾青素对铜离子诱导的RWPE-1和PC-3细胞凋亡的影响;荧光分光光度法测定了虾青素对铜离子诱导的活性氧自由基(ROS)产生的影响;采用罗丹明123(Rh123)染色检测虾青素对铜离子诱导的细胞线粒体膜电位(MMP)变化的影响;采用试剂盒测定了虾青素对铜离子存在下丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活性变化的影响。结论:结果表明,铜离子能诱导RWPE-1和PC-3细胞凋亡,并伴随细胞内ROS和MDA含量升高;虾青素处理可显著降低RWPE-1细胞中MDA含量,升高线粒体膜电位,并保持ROS含量稳定;虾青素处理可降低PC-3细胞中SOD、GSH-Px和CAT的活性,而对RWPE-1细胞则作用相反。因此,虾青素处理能有效降低铜离子对RWPE-1细胞引起的损伤,而通过降低抗氧化酶活性加剧铜离子对PC-3细胞的损伤。