亚甲基蓝及其代谢物在异育银鲫体内分布及消除规律的研究

Tissue distribution and elimination of methylene blue and its metabolites in allogynogenetic crucian carps (Carassius auratus gibelio)

以15 mg·L^(-1)的亚甲基蓝浸泡异育银鲫(Carassius auratus gibelio)成鱼0.5 h后将其饲养于实验室水族箱中,研究了常规用药条件下亚甲基蓝及其代谢物在异育银鲫各组织中的分布及消除规律。采用高效液相色谱法检测亚甲基蓝及其代谢物在异育银鲫组织中的浓度,用DAS 3.0.5药物与统计软件对数据进行分析。结果表明:血液中亚甲基蓝、天青B、天青A达峰时间Tmax分别为第0.083、第0.5和第8小时,峰浓度值Cmax分别为143.436μg·L^(-1)、110.770μg·L^(-1)和8.444μg·L^(-1)。亚甲基蓝、天青B、天青A在异育银鲫4种组织中的浓度由高到低的顺序均为肝胰脏>肾脏>肌肉>皮肤,其中异育银鲫肌肉和皮肤组织易蓄积亚甲基蓝及代谢物,至第64天仍可以检测到,组织中未检测到天青C。

We investigated the distribution and elimination rules of methylene blue and its metabolites in allogynogenetic crucian carps（ Carassius auratus gibelio） which were raised in aquariums after 0. 5-hour bath treatment of 15 mg·L（-1）methylene blue. The concentrations of methylene blue and its metabolites in the main tissues were simultaneously detected by HPLC. The data were analyzed using pharmacokinetics software DAS 3. 0. 5. The results show that peak concentrations of methylene blue, azure B and azure A were143. 436 μg·L（-1）,110. 770 μg·L（-1）and 8. 444 μg·L（-1）at 0. 083 thhour,0. 5thhour and 8thhour,respectively. Four kinds of tissue concentrations of methylene blue,azrue B and azrue A from high to low were hepatopancreas,kidney,muscle and skin. Methylene blue,azrue B and azrue A were likely to accumulate in muscle and skin,which still could be detected until 64 thday. Azrue C was not detected in tissues of allogynogenetic crucian carp.