一种体视显微镜聚焦一致性的判定方法

A method for determined the focusing consistency of stereo optical microscope

针对立体显微图像在同一位置双目清晰度不同的问题,提出了一种体视显微镜聚焦一致的性判定方法。使用连通区域分割方法对不同深度标定板图像进行分割,提取圆点中心,选取清晰度评价区域;使用聚焦测度对不同深度图像进行清晰度评价,得到清晰度-深度关系曲线,利用曲线拟合求取曲线峰值得出深度信息;利用左右目圆点中心的三维坐标分别拟合出左右全聚焦平面,通过测量左右全聚焦平面夹角以及评价区域中左右目圆点中心距离来分析聚焦性。结果表明,能够有效地判定体视显微镜聚焦的一致性。

For microscope stereo image clarity may be different at the same location, a method for determined the fo- cusing consistency of stereo optical microscope is proposed. Dot centers of different depth calibration board are extracted with region grown segmentation algorithm and regions to be evaluated are selected. Focus measure function is used to e- valuate the selected image regions and definition-depth curve is obtained. The curve peak is got by curve fitting technique. The depth is draw and 3D depth measurement is completed. Using the left and right eye dots in the centers of three-di- mensional coordinates are fitted around the focal plane. Focus plane angle measured and distance of dots center focusing are used to analysis the focusability. Experimental results show that the method can effectively determine the focusing consistency of stereo optical microscope.