水杨酸浓硫酸比色法测定浒苔中硝酸盐含量

Salicylic acid-concentrated sulfuric acid colorimetry method for determination of nitrate content in Ulva prolifera

浒苔是我国绿潮的主要肇事物种,测定其体内硝酸盐含量对研究浒苔对硝酸盐的同化和吸收有着重要意义。本文采用水杨酸浓硫酸比色法对浒苔体内硝酸盐含量进行了测定,并对该方法中样品预处理、显色剂使用量及工作曲线选择等步骤进行了相应的探讨及优化,使其更适用于测定浒苔体内的硝酸盐含量,同时确定了该方法的检出限、精确度和准确度。研究结果表明本文所确定的方法适用于浒苔样品中硝酸盐含量的快速测定。

Green-tide events occurred in the Yellow Sea successively in recent years. The marine macrophyte Ulva prolifera is the dominant green-tide-forming seaweed. It is necessary to study the nitrogen contents in the algal tissue, which contributes to knowing the uptake and assimilation of nitrate by Ulva prolifera. In this study, the method of salicylic acid-concentrated sulfuric acid colorimetry was employed to investigate the nitrate contents in Ulva prolifera. The influenced factors, including sample pretreatment, the amount of chromogenic reagent addi- tion and the selection of performance curve, were examined and optimized during the determinations. The detection limit, precision and accuracy of this method were also established simultaneously. Our results indicated that this method was applicable for the fast examination of the nitrate contents in the algal tissue.