达氏鲟gdf11基因cDNA的克隆及组织表达特征分析

Cloning and expression pattern of gdf11 cDNA in Acipenser dabryanus

本研究克隆了达氏鲟(Acipenser dabryanus)生长转化因子gdf11(growth differentiation factor 11)基因cDNA。达氏鲟gdf11基因cDNA序列全长1 298 bp(不包括Poly A),开放阅读框为1 191 bp,编码396个氨基酸,5非编码区长19 bp;3非编码区长88 bp。通过Signal P软件预测含有N端21aa的信号肽。组织表达特征分析结果显示,gdf11在7种组织中均有表达,在眼和鳃中的表达量较高;不同水流条件下,鳃和心脏gdf11的表达量有显著性差异,静水中鳃gdf11表达量是流水(流速27.0±3.0 cm/s)的4.70倍,而心脏中的表达量是流水的2.72倍。这暗示了gdf11可能在呼吸代谢中发挥功能。

The full- length cDNA of the gdf11（ growth differentiation factor 11） gene from Acipenser dabryanus was cloned. The gdf11 gene cDNA is 1 298 bp in size and has a 1 191 bp ORF（ open reading frame） encoding a putative protein of 396 amino acid residues,and 5 and 3 non- coding region were 19 bp and 88 bp respectively. By Signal P prediction,it was found that A. dabryanus gdf11 protein contained a 21 amino acid sid it was expressed highlygnal peptide. The RT- q PCR analysis showed that gdf11 was existed in all the examined tissues,an in the eyes and gills. Under different flow conditions,significant differences were found in the gdf11 expression level of the gills and heart,the expression of gdf11 gene in the gill and heart of A. dabryanus in the static water group is 4. 70 times and 2. 72 times of those in the flow enriched water group（ 27. 0 ± 3. 0 cm/s）. These implied gdf11 might have functions in the respiratory metabolism.