摘要
目的摘探讨白藜芦醇对力竭运动大鼠心肌细胞氧化应激、凋亡影响及机制。方法成年雄性SD大鼠随机分为5组:对照组、力竭运动组、白藜芦醇50、100、200 mg/kg组,检测大鼠心肌组织缺血缺氧面积、心肌组织超氧化物歧化酶(SOD)、丙二醛、Ca^(2+)-ATPase含量,TUNEL法检测心肌细胞凋亡率,Western blot法检测心肌细胞Bax和caspase-3蛋白表达。结果与对照组比较,力竭运动组大鼠心肌缺血缺氧面积[(112.27±48.27)μm^2]与心肌组织丙二醛含量[(3.98±0.75)nmol/mgpro]明显升高、心肌组织SOD活性[(30.54±4.29)U/mgpro]与Ca^(2+)-ATPase活性[(0.32±0.03)mol/(mg·h)]明显下降、心肌细胞凋亡指数[(23.4±2.3)%]与心肌组织Bax、caspase-3蛋白表达[(1.28±0.32)、(1.59±0.12)]明显升高;与力竭运动组比较,200 mg/kg白藜芦醇组大鼠心肌缺血缺氧面积[(22.12±10.22)μm^2]与心肌组织丙二醛含量[(1.65±0.11)nmol/mgpro]明显减少、心肌组织SOD、Ca^(2+)-ATPase活性[分别为(69.57±9.02)U/mgpro、(0.72±0.11)mol/(mg·h)]明显升高、心肌细胞凋亡指数[(10.4±1.3)%]与心肌组织Bax、caspase-3蛋白表达[(0.36±0.11)、(0.62±0.13)]明显降低(P<0.01);呈明显剂量效应关系。结论白藜芦醇可有效缓解大鼠力竭运动所致氧化应激状态,其机制可能与降低心肌组织Bax、caspase-3表达水平,减少心肌细胞凋亡有关。
Objective To explore protective effects of resveratrol (RIGS) on oxidative stress, carchomyocyte apoptosis in exercise-exhauted rats. Methods Fifty Sprague-Dawley (SD) specific pathogen-free (SPF) adult male rats were randomly divided into five groups (quiet control, exhaustive-exercise, and three RES treatment groups at the dosages of 50,100,200 mg/kg). Superoxide dismutase ( SOD), malondialdehyde ( MDA), and calcium-activated adenosine triphosphatase (Ca2 +-ATPase) in heart tissues of all the rats were measured. Cell apoptosis was detected using terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) method. The expression of B cell lymyhoma/leukemia-2- associated X protein (Bax) and caspase-3 protein in the heart tissues were measured using Western blot. Results The myocardial tissues of exercise-exhausted rats showed significantly higher hypoxic-ischemic area ( 112. 27 ± 48.27 μm2 ), cell apoptosis index ( 10. 4 ±1.3 ) % , MDA ( 3.98 ± 0. 75 nmol/mgpro), Bax ( 1.28 ± 0. 32), and caspase-3 ( 1.59 ± 0. 12) and lower SOD (30. 54 ± 4. 29 U/mgpro ), Ca2 + -ATPase ( 0. 32 ± 0.03 mol/mg . h) compared to those of the quiet control rats; the myocardial tissues of the rats treated with RES of 200 mg/kg manifested significantly lower hypoxic-ischemic area (22. 12 ± 10. 22 μm2 ), cell apoptosis index (23.4 ± 2. 3 ) %, MDA ( 1.65 ± 0. 11 nmol/mgpro), Bax (0. 36 ±0. 11) ,and caspase-3 (0. 62 ±0. 13) and higher SOD (69. 57 ±9.02 U/mgpro) ,Ca2+ -ATPase (0. 72± 0. 11 mol/mg. h ) compared to those of the exercise-exhausted rats ( all P 〈 0.01 ). Conclusion RES can reduce oxidative stress in exercise-exhausted rats and the effect may be related to the decreased Bax and caspase-3 expression in myocardial tissues and inhibited cardiomyocyte apoptosis.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2017年第2期214-217,共4页
Chinese Journal of Public Health