基质辅助激光解吸电离飞行时问质谱(MALDI-TOFMS)分析在丝状真菌实验诊断中的应用

Application of Matrix-assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS) in the Laboratory Diagnosis of Filamentous Fungi

基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF MS)是一种可以对多种微生物(包括细菌、酵母和丝状真菌)进行常规鉴定的强有力工具。具有快速、准确、高通量及成本低廉等特点,逐渐被用于丝状真菌感染的实验室诊断中。在丝状真菌的MALDI-TOF MS分析中,从样本制备到获得准确的鉴定结果,每个步骤都决定了医技人员能否及时为临床医生提供准确结果。现就待测样品的制备及MALDI-TOF MS技术对丝状真菌分析(包括鉴定、分型、抗真菌敏感性测试方面)的应用及前景作一系统综述,也将对其一些未来发展进行展望。

Matrix-assisted laser desorption / ionization time-of-flight mass spectrometry （MALDI-TOF MS） is a powerful tool for routine identification of a variety of microorganisms, including bacteria, yeast and filamentous fungi. Fast, accurate, high-throughput and low-cost characteristics,and gradually used for filamentous fungal infection in the laboratory diagnosis. In the MALDI-TOF MS analysis of filamentous fungi, from sample preparation to obtaining accurate identification results, each step determines whether the medical technician can provide accurate results for the clinician. This review will provide a systematic overview of the applications and prospects of filamentous fungal analysis （including identification, typing,and an- tifungal susceptibility testing）from preparation of the test sample to the MALDI-TOF MS technique,as well as a review of some of its future developments.