外周血异常糖链糖蛋白检测对非小细胞肺癌患者治疗监测的意义

Significance of Tumor Abnormal Protein in Peripheral Blood in the Therapeutic Monitoring of Non-Small Cell Lung Cancer

目的观察肿瘤异常糖链糖蛋白(TAP)对非小细胞肺癌(NSCLC)患者治疗监测的意义。方法选取临床确诊为NSCLC的患者30例,采集其治疗前、治疗后半个月、一个月、三个月、六个月外周静脉血并涂片,用凝集法检测TAP,以最大凝聚物面积为衡量指标,评估其对NSCLC治疗监测的意义。同时收集30例健康体检者血作为对照组。.结果治疗前NSCLC患者TAP阳性率为86.67%,健康体检者TAP阳性率为3.33%,差异有统计学意义(x2=140.3,P<0.01);Ⅲ~Ⅳ期NSCLC患者TAP凝聚物面积[411(89,562)mm2]高于Ⅰ~Ⅱ期NSCLC患者[267(31,407)mm,差异有统计学意义(H=18.91,P<0.05);两组患者治疗后TAP凝聚物面积均开始降低,Ⅰ~Ⅱ期患者治疗一个月后凝聚物面积与治疗前差异有统计学意义(Z=-3.21,P<0.01),Ⅲ~Ⅳ期患者治疗半个月后TAP凝聚物面积与治疗前差异有统计学意义(Z=-2.78,P<0.05)。治疗三个月后,两组凝聚物面积均达最低水平,Ⅰ~Ⅱ期患者平均凝聚物面积下降至治疗前的21%,Ⅲ~Ⅳ期患者下降至治疗前的37%,且Ⅰ~Ⅱ期患者TAP凝聚物面积下降幅度高于Ⅲ~Ⅳ期患者,差异有统计学意义(x^2=6.22,P<0.05)。结论外周静脉血TAP检测NSCLC敏感性较高,可用于NSCLC患者的疗效监测,评估治疗效果。

Objective To observe the significance of tumor abnormal protein （TAP） in the therapeutic monitoring of non- small cell lung cancer （NSCLC）. Methods Peripheral blood from 30 NSCLC patients were collected to make smears at the moment of pre-treatment, half a month,one month, 3 months and 6 months after therapy. TAP was detected by coacervation method. The maximum area of condense was applied to estimate the level of TAP. Thirty healthy subjects were chose as con- trol group. Results The positive rate of TAP in NSCLC patients was 86. 67% ,and 3.33% of healthy subjects were positive in TAP. The difference was statistically significant （χ2=140.3 ,P〈0.01）. The condense area of TAP in patients withⅢ-ⅣV stage of NSCLC [411（89,562）mm2] was significantly higher than those withⅠ-Ⅱ stage NSCLC [-267（31,407）mm2 ]. The condense areas in both of two groups went down after treatment. A significant difference of condense area appeared inⅠ-Ⅱstage of NSCLC patients a month after therapy as well as Ⅲ-Ⅳstage of NSCLC patients half a month after treat- ment. The condense areas went to their lowest level 3 months after therapy. FoⅠ-Ⅱ stage patients,the condense area fell to 21 % compared to that before treatment, but for patients with Ⅲ-Ⅳ stage of NSCLC,37 % of pre-treatment condense ar- ea were detected. The Ⅰ-Ⅱ stage of NSCLC patients had a greater decrease in condense area of TAP than theⅢ-Ⅳ stage patients by 3 months after treatment （χ2 = 6.22, P〈0.05）. Conclusion Detection of TAP in peripheral blood had a high sensitivity for NSCLC,and is able to monitoring the treatment effect.