XN-9000全自动血液细胞分析仪有核红细胞计数与手工方法验证的探讨

Evaluation of the Nucleated Red Blood Cells Count of Sysmex XN-9000 Automatic Hematology Analyzer and the Validation of Manual Method

目的探讨日本Sysmex公司生产的XN-9000全自动血液细胞分析流水线有核红细胞(nucleated red blood cell,NRBC)计数,与手工方法进行比较,验证仪器计数结果的准确性。方法对60例全自动血液细胞分析流水线检测出NRBC大于1%的患者的血液标本,制作血液涂片染色后通过传统的显微镜手工方法镜检,验证仪器结果。结果 60例临床标本的NRBC与显微镜计数的结果比较,所有检测值的可信度分析均在可信范围内;相关性比较中,NRBC(%)在1~10和>10两组的仪器与手工方法的相关系数(r)分别为0.972 1和0.996 2,差异均无统计学意义(P>0.05)。结论无论NRBC值的高低,XN-9000全自动血液细胞分析流水线检测NRBC计数,与手工方法结果比较,均在可信范围内,相关性也较好。仪器检测的NRBC准确可靠,可应用于临床标本的检测。

Objective To evaluate the nucleated red blood cell （NRBC） count of Sysmex XN 9000 automatic hematology anal- ysis lines comparing with manual method,and verify the accuracy of the analyzer results. Methods 60 blood samples with more than 1 % of NRBCs detected by XN-9000 were counted NRBCs by traditional manual microscopy in blood smears,and verified the analyzer results. Results According to the reliability analysis,the results of total 60 samples were all within the range of the reliabilityl correlation analysis showed that correlation coefficient （r） of group NRBC （ % ） 1 -10 and 〉 10 were 0. 972 1 and 0. 996 2,respectively. There were no significant differences between them （P〉0.05）. Conclusion Compared with manual method,the results of NRBC count of XN-9000 were within the range of reliability, and showed good correla tion. The analyzer test results of NRBC were accurate and reliable and could be applied to the detection of clinical samples.