补中益气汤“要药”配伍对脾虚大鼠胃肠推进及血浆Ghrelin、NO和VIP含量的影响

Effect of Key Herbs Compatibility in Buzhong Yiqi Decoction on Gastrointestinal Propulsion and Plasma Content of Ghrelin,Nitric Oxide and Vasoactive Intestinal Peptide in Spleen Deficiency Rats

目的:观察补中益气汤"要药"配伍对脾虚胃肠动力障碍大鼠胃肠推进及血浆中Ghrelin,NO和VIP含量的影响,探讨补中益气汤治疗脾虚胃肠动力障碍的机制及其"要药"配伍的科学性。方法:60只SD大鼠随机抽取10只为正常对照组,剩余复制脾虚胃肠动力障碍模型,造模结束后随机分为模型组、莫沙必利组、补中益气汤组、补中益气汤去升柴组和升柴组(每组10只),分别灌胃给予相应药物治疗14 d,给药结束后获取标本测定胃肠推进率和血浆Ghrelin,NO,VIP的含量。结果:(1)与正常组相比,模型组大鼠的胃排空率明显升高(P<0.05),而小肠推进率显著下降(P<0.01);与模型组相比,莫沙比利组、补中益气汤组和升麻柴胡组的胃排空率显著升高(P<0.05,P<0.01,P<0.01),莫沙比利组、补中益气汤组和升柴组的小肠推进率明显升高(P<0.05,P<0.01,P<0.01);去升柴组胃排空率和小肠推进率与模型组相比无显著性差异(P>0.05)。(2)与正常组相比,模型组Ghrelin显著下降(P<0.01),NO和VIP显著升高(P<0.05,P<0.01);经药物干预后,莫沙必利组,补中益气汤组和升麻柴胡组Ghrelin相比模型组有显著升高(P<0.05,P<0.05,P<0.05),去升柴组没有显著变化(P>0.05);莫沙必利组,补中益气汤组和去升柴组NO相比模型组有显著降低(P<0.01,P<0.01,P<0.01),升柴组没有明显改变(P>0.05);莫沙必利组,补中益气汤组和去升柴组VIP相比模型组有显著降低(P<0.05,P<0.05,P<0.01),升柴组VIP没有显著变化(P>0.05)。结论:脾虚胃肠动力障碍模型大鼠的胃排空亢进和小肠推进率下降,血浆Ghrelin下降,NO和VIP含量明显升高;补中益气汤具有良好的促胃肠动力作用,其机制为升高脾虚大鼠的Ghrelin,降低NO和VIP含量;单纯升麻柴胡配伍对部分指标有改善作用,提示升麻柴胡为补中益气汤的"要药",升麻柴胡配伍有其科学性。

Objective： To explore the action mechanism of key herbs compatibility in Buzhong Yiqi Decoction （BZYQD） on gastrointestinal motility disorder in spleen deficiency rats and verify its seientificity of Cimicifugae Rhizoma （Shengma） and Bupleuri Radix（Chaihu） combination in BZYQD through observing the effect of key herbs compatibility in BZYQD on gastrointestinal propulsion and blood plasma content of ghrelin, nitric oxide （NO） and vasoactive intestinal peptide（VIP） in spleen deficiency rats. Method： Ten rats were selected randomly from 60 SD rats as control group. The other rats were used to duplicate gastrointestinal dysmotility model, and then randomly divided into the model group （ MODG）, mosapride group（ MPG）, Buzhong Yiqi Decoction group（ BZYQDG）, BZYQD ＋ Shengchai group （MUSCG） and Shengehai group（SHCHG）. Gastrointestinal propulsion and plasma content of Ghrelin, NO and VIP were determined after administration of corresponding medicinal for 2 weeks and then the rats were sacrificed for sampling and determination of parameters. Result ： （ 1 ） Compared with control group, gastric emptying rate of MODG increased significantly （ P 〈 0.05 ） and the intestinal propulsion rate decreased significantly（ P 〈 0.01 ）. Compared with MODG, gastric emptying rate of MPG, BZYQDG, SHCHG increased significantly（ P 〈 0. 05, P 〈 0. 01, P 〈0.01 ） and the intestinal propulsion rate increased significantly （ P 〈 0. 05, P 〈 0. 01, P 〈 0.01 ）. （2） Compared with control group, the blood plasma content of ghrelin of MODG declined obviously（ P 〈0.01 ） while the contents of NO and VIP increased obviously （ P 〈 0.05, P 〈0.01 ）. After administration, compared with MODG, the content of ghrelin of MPG, BZYQDG and SHCHG increased significantly （ P 〈 0. 05, P 〈 0. 05, P 〈 0.05） while no obvious change was seen in MUSCG （ P 〉 0.05）. Compared with MODG, the contents of NO of MPG, BZYQDG and MUSCG significantly reduced （ P 〈 0. 01, P 〈 0. 01, P 〈0.01 ） while no obvious change was seen in SHCHG （ P 〉0.05）. Compared with MODG, the contents of VIP of MPG, BZYQDG and MUSCG reduced significantly （ P 〈 0. 05, P 〈 0. 05, P 〈0.01） while no obvious change was seen in SHCHG （ P 〉 0. 05 ） . Conclusion ： The gastric emptying rate increases significantly while the intestinal propulsion rate decreases significantly. BZYQD has obvious effect on promoting gastrointestinal dynamics through the mechanism of increasing ghrelin content and decreasing NO and VIP contents of rats with spleen deficiency. The key herbs （Rhizoma Cimicifugae and Radix Bupleuri） just partially act on above mentioned parameters, indicating that Rhizoma Cimicifugae and Radix Bupleuri are the key herbs of BZYQD. Rhizoma Cimicifugae and Radix Bupleuri compatibility in BZYQD has its inherent seientificity.