抗PUF60抗体在系统性红斑狼疮中的分布及临床意义

The prevalence of anti-PUF60 autoantibodies in patients with systemic lupus erythematosus

目的:检测系统性红斑狼疮(SLE)患者血清中抗PUF60抗体水平,探讨该抗体阳性的SLE患者的临床和实验室特征。方法:选取我院收治的SLE患者104例和健康对照163名。采用ELISA法测定上述人群血清中抗PUF60抗体,部分抗体阳性血清采用免疫印迹法进一步验证。结果 :血清抗PUF60抗体在SLE中的阳性率为17.3%(18/104)。抗PUF60抗体阳性的SLE患者发病年龄[21(18,27)岁]低于抗体阴性组[29(20,37)岁](P=0.007),脱发的发生率[72.2%(13/18)]高于抗体阴性组[40.7%(35/86)](P<0.05)。抗PUF60抗体阳性组中补体C4降低、外周血B1细胞数目升高的患者比例和抗ds DNA抗体、抗Sm抗体及抗SSB抗体的阳性率显著高于抗PUF60抗体阴性组(P<0.05)。结论 :本研究首次对我国SLE患者血清中抗PUF60抗体进行检测,发现抗PUF60抗体是SLE的相关性抗体,该抗体阳性者具有独特的临床表型。

Objective：To determine the sera levels and clinical significance of anti-PUF60（poly-U-binding factor 60 k Da）antibodies in patients with systemic lupus erythematosus（SLE）.Methods：Sera from 104 Chinese patients with SLE and 163 healthy controls were collected.The anti-PUF60 antibodies were measured by enzyme linked immunosorbent assay.Four cases positive sera were further examined by immunoblot assays.Results：The positive rate of sera anti-PUF60 autoantibodies in patients with SLE was 17.3%（18/104）.The antiPUF60-positive patients had significantly younger age [21（18,27）vs 29（20,37）years old,P=0.007]and higher incidence of alopecia [72.2%（13/18） vs 40.7%（35/86）,P〈0.05]than the anti-PUF60-negative patients.Compared with anti-PUF60-negative patients,the occurrences of hypocomplementemia,elevated B1-cell subpopulations were much higher in anti-PUF60-positive patients than in anti-PUF60-negative patients（P〈0.05）.Meanwhile,the positive rates of anti-ds DNA,Sm,SSB were higher in patients with positive anti-PUF60 antibodies（P〈0.05）.Conclusion：We found that anti-PUF60 antibody was a new valuable SLE-associated antibody in Chinese,which related to specific clinical manifestations.