细胞培养实验中细胞系鉴定及质量控制重要性探讨

Importance of Cell Lines Identification and Quality Control During Cell Culture

目的以脑胶质瘤细胞系(U87)为例,探讨对在细胞培养实验中所使用的细胞系进行鉴定和质量控制的重要性。方法对三株不同来源的U87细胞系进行STR分型检测,并结合显微镜下形态学特征,判断所测细胞系是否为真正的U87细胞系及是否发生交叉污染。结果通过细胞STR分型结果与ATCC、DSMZ中STR数据库对比,得出U87-1细胞分型与ATCC中U-87 MG Glioblastoma-Astrocytoma Human100%匹配;U87-2细胞分型与DSMZ数据库中U-87MG100%匹配;U87-3未得到扩增产物,不能进行分型,原因是该细胞株为非人源细胞。结论细胞培养实验中所使用细胞系的鉴定对于后续研究具有重要意义,可使用STR分型检测技术在细胞系培养过程中进行质量控制,对其进行准确鉴定,并排查是否存在细胞交叉污染。

Objective To discuss the importance of cell identification and quality control in the process of cell lines culturing by using glioma cell lines（ U87） as a model. Methods Three glioma cell lines of U87 were examined by short tantem repeat（ STR） profiling. M orphological characteristics were reviewed to determine whether the cell lines were real U87 cell lines and whether they had cross contamination. Results The results of STR typing were compared with ATCC and DSM Z STR database. U87-1 was matched 100% to ATCC standard cell line marked as U-87 M G Glioblastoma-Astrocytoma Human. U87-2 was matched 100% to DSM Z standard cell line marked as U-87 M G. U87-3 could not be amplified and could not be matched. The reason might be that the cell lines were non-human cells. Conclusion The identification of cell lines used in cell culture experiments has great significance for the subsequent studies, We can use STR profiling technology for the quality control of celllines, accurate identifying cell lines and screening cross contamination.