丹枝饮对盆腔炎性疾病后遗症小鼠体内炎性因子的影响

Effect of Danzhi decoction on serum levels of inflammatory factors in murine model with sequelae of pelvic inflammatory disease

[目的]观察丹枝饮对盆腔炎性疾病后遗症(SPID)小鼠血清炎性因子白介素-17A(IL-17A)、白介素-17F(IL-17F)、单核细胞趋化蛋白-1(MCP-1)、巨噬细胞炎症蛋白-2(MIP-2)表达水平的影响,探讨盆腔炎性疾病后遗症的发病机制及丹枝饮治疗SPID的药理作用机制。[方法]将48只SPF级BALB/c小鼠随机分为正常组、模型组、阿司匹林组[20.55 mg/(kg·d)]、丹枝饮小剂量组[7.91 g/(kg·d)]、丹枝饮中剂量组[15.82 g/(kg·d)]、丹枝饮大剂量组[31.64 g/(kg·d)],每组8只。其中模型组、阿司匹林组、丹枝饮小、中、大剂量组采用宫腔种植鼠衣原体复制SPID小鼠模型,造模后第6周开始灌胃给药3个动情周期(15 d)后,检测各组小鼠脸谱疼痛评分(MGS),血清IL-17A、IL-17F、MCP-1、MIP-2的表达水平。[结果]与模型组相比较,阿司匹林组、丹枝饮小、中、大剂量组MGS评分,血清IL-17A、IL-17F、MCP-1、MIP-2的表达水平均有显著降低(P<0.01),且丹枝饮中剂量组在各项指标的降低程度上均优于阿司匹林组、丹枝饮小剂量组及丹枝饮大剂量组(P<0.05)。[结论]盆腔炎性疾病后遗症可以引起炎性因子IL-17A、IL-17F、MCP-1、MIP-2的表达水平的增高,丹枝饮可以有效的减轻SPID小鼠脸谱疼痛评分,降低SPID小鼠血清炎性因子IL-17A、IL-17F、MCP-1、MIP-2的表达水平,从而达到治疗SPID的作用。

[Objective] To investigate the effect of Danzhi Dection（DZD） on serum levels of interleukin-17A（IL-17A）, interleukin-17F（IL-17F）, monocyte chemoattractant protein-1（MCP-1） and macrophage inflammatory protein-2（MIP-2） in murine model with sequelae of pelvic inflammatory disease. To explore the pathogenesis of sequelae of pelvic inflammatory disease and explain the pharmacological action of Danzhi Dectionin treating SPID. [Methods]48 BALB/c mice were randomly divided into normal control group, model group, aspirin（ASP） group [20.55 mg/（kg·d）],DZD low-dose group [7.91 mg/（kg·d）], DZD mid-dose group [15.82 mg/（kg·d）] and DZD high-dose group[31.64 mg/（kg·d）], 8 mice in each group. The mice of the model group, ASP group, DZD low-dose group, DZD middose group and DZD high-dose group were given injected Chlamydia muridarum into the uterine cavity to induce SPID. After modeling of 6 weeks, each group were given medicine for 15 d. The mouse grimace scale（MGS）, the serum levels of IL-17 A, IL-17 F, MCP-1 and MIP-2 of mice were detected. [Results] To compare with model group,the MGS, the serum levels of IL-17 A, IL-17 F, MCP-1 and MIP-2 were significantly decreased in ASP group, DZD low-dose group, DZD mid-dose group and DZD high-dose group（P〈0.01）. And the effect of DZD mid-dose group was better than other treatment groups（P〈0.05）. [Conclusion] SPID can enhance the expression levels of IL-17 A,IL-17 F, MCP-1 and MIP-2, Danzhi Dection can effective reduce the MGS, the serum levels of IL-17 A, IL-17 F, MCP-1 and MIP-2 in SPID mice. Danzhi Dection is the better choice to treat SPID.