NF-κB在内毒素血症大鼠肝损伤中的变化及意义

Study On the Changes and Its Significance of NF-κB on Liver Injury for the Rats With Endotoxemia

目的观察NF-κB在内毒素血症大鼠肝损伤中的变化,分析内毒素血症中NF-κB表达的意义。方法选取40只健康Wistar大鼠,随机分为肝损伤组和对照组,各20只,肝损伤组大鼠建立内毒素血症肝损伤模型,在建模前后观察组间NF-κB表达水平的变化。结果在造模后3 h时、6 h、12 h时,内毒素组NF-κB表达水平[(14.83±1.65)D、(21.35±2.57)D、(31.63±3.57)D]和肝损伤程度[(1.14±0.87)分、(2.06±1.03)分、(3.02±1.35)分]高于对照组,差异有统计学意义,P<0.05。结论内毒素血症肝损伤大鼠肝组织内NF-κB表达升高,考虑NF-κB可能参与了肝损伤过程。

Objective To observe the changes of NF-κB on liver injury for the rats with endotoxemia, to analyze and the significance of NF-κB for endotoxemia. Methods 40 healthy wistar rats were selected and randomly divided into liver injury group and control group. There were 20 rats in each group. The endotoxemia liver injury model was established in liver injury group. The changes of NF-κB expression level were observed before and after modeling. Results After modeling for 3 hours, 6 hours,12 hours,NF-κB expressions in endotoxin Group were [（14.83±1.65） D,（21.35±2.57） D,（31.63±3.57） D] and the degrees of liver injury were [（1.14±0.87） points,（2.06±1.03） points,（3.02±1.35） points], they were significantly higher than the control group, the differences were significant between the groups, P〈0.05. Conclusion NF-κB expression is significantly increased in liver tissue of the liver injury rats with endotoxemia,NF-κB may be the factor for liver injury.