介孔生物玻璃/丝素蛋白复合多孔海绵的结构及止血性能研究

Study on structure and hemostatic property of mesoporous bioglass/silk fibroin composite sponge

采用冷冻干燥法制备了介孔生物玻璃(M58S)/丝素蛋白(SF)复合多孔海绵,采用透射电镜(TEM)和场发射扫描电镜(FESEM)等观察M58S和复合多孔海绵的形貌和结构特征,通过体外凝血实验、大鼠肝脏止血模型和体外细胞毒性实验评价复合多孔海绵的止血性能和细胞毒性。TEM显示M58S内部具有规则的纳米级介孔孔道,排列高度有序,比表面积达400m^2/g,平均孔径为7.3nm;FESEM显示复合海绵呈现多孔沟壑状结构,孔隙率>80%;APTT和PT测试结果表明该复合多孔海绵主要通过作用于血液的内源性凝血系统途径促进凝血;小鼠肝脏止血模型显示当M58S含量>15%时,复合多孔海绵的止血效果较好且优于明胶海绵,有望用作体外快速止血材料。

The mesoporous bioglass/silk fibroin （M58S/SF） composite sponge was prepared by freeze drying method. The morphology and structure of the composite sponge was characterized by TEM, FESEM, etc. The hemostatic property and cytotoxicity of the composite sponge were measured by in vitro coagulation method, rat liver injury model and in vitro cytotoxicity experiments. TEM images verified a highly ordered nano channel structure in M58S. The BET surface area and pore size of M58S were calculated to be 400 m2/g and 7.3 nm, respectively. FESEM images showed that the micromorphology of composite sponge were porous gully shape. The porosity of the composite sponge was more than 80 %. APTT and PT measurements reflected that the composite sponge mainly activated the intrinsic pathway of coagulation cascade. The result of murine hepatic injury model showed that the hemostatic effect of the composite sponge was better than that of gelatin sponge when the content of M58S was more than 15%. In this study, the M58S/SF composite sponge may be a potential biomaterial for rapid hemostasis.