Kingfisher FLEX自动工作站在批量提取脱落细胞检材DNA中的应用

Batch Extraction of DNA from Epithelial Cells by the Automatic Workstation of Kingfisher FLEX

目的建立基于自动工作站的大批量提取实验室日常检案受理的脱落细胞检材的DNA的方法。方法针对不同的脱落细胞检材分别采用直接剪取法、两步擦拭法、胶带粘取法或负压吸附法提取脱落细胞,应用Kingfisher FLEX自动工作站,结合使用LC-超微磁珠DNA提取试剂盒和Power Plex?18D试剂盒,对现场脱落细胞检材DNA进行批量提取和扩增,计算成功检出STR基因座的检材比率。结果应用该方法,成功检出STR基因座的检材比率为59.7%。结论该方法稳定、高效、检出率高,可用于脱落细胞检材DNA的批量提取。

Objective To design a method for batch extraction of DNA from epithelial cells in large quantities by an automatic workstation. Methods Different choices（direct cutting, 2-step wiping, adhesive-tape sticking or negativepressure adsorption） were carried out to collect sloughed epithelial cells from different forms of samples. DNA was in batch extracted from the above collected epithelial cells with LC-Microbeads DNA extraction kit combined with Kingfisher FLEX automatic workstation, and then amplified with Power Plex？18D kit. The success ratio of STR typing was calculated from the electrophoresis of the amplified DNA. Results The success ratio of STR typing is 59.7%. Conclusion This method is stable and efficient, yet to be improved of its detection rate, suitable for batch extraction of DNA from epithelial cells in large quantities.