用DNA条形码技术对市售花胶进行基原鉴定

Identification of Original Species of Fish Maw by DNA Barcoding

目的:运用DNA条形码技术确定广州市售花胶的物种来源。方法:用自行设计的COⅠ引物对11份市售花胶样品进行PCR扩增、测序,将所得序列构建UPGMA系统发育树,进行聚类分析;用BOLD(The Barcode of Life Data Systems)系统的物种鉴定引擎,与数据库中已有鱼类序列进行比对,鉴定出各样品的基原。结果:11份市售花胶样品用自行设计的引物均可实现扩增,条带清晰、稳定,扩增和测序成功率均为100%;BOLD鉴定结果显示,11份样品来源于3科5种鱼类,UPGMA聚类分析对结果进行了确证。结论:DNA条形码技术与BOLD鉴定系统相结合,可对市售花胶进行准确的基原鉴定。

Objective： To identify the original species of fish maw sold in Guangzhou market by DNA barcoding technology. Methods： Mitochondrial cytochrome C subunit Ⅰ（ COⅠ） gene fragment of eleven fish maw samples were amplified and sequenced with the self-designed primers. UPGMA phylogenetic tree were constructed for clustering analysis. The species origin of each sample was identified with the identification engine provided in the Barcode of Life Data Systems（ BOLD）. Results： The self-designed primers were effective in fish maw COⅠ amplification and sequencing,with success rates both of 100%. BOLD identification and UPGMA clustering analysis indicated the fish maw samples were derived from five fish species of three families. Conclusion： DNA barcoding combined with BOLD identification system can accurately identify the species origin of commercial fish maw.