七叶皂苷钠阻断JAK-1/STAT-1信号诱导BGC-823及AGS细胞凋亡

Sodium Aescinate Induced Apoptosis of BGC-823 and AGS Cells by Inhibiting JAK-1/STAT-1 Signaling Pathway

目的:探讨七叶皂苷钠诱导胃腺癌BGC-823细胞及胃癌AGS细胞凋亡的机制。方法:采用CCK-8法检测七叶皂苷钠作用后胃癌细胞活力的变化;倒置显微镜下观察胃癌细胞的形态变化;荧光倒置显微镜观察DAPI单染后细胞核形态的改变;流式细胞术检测细胞凋亡率;Western bloting检测JAK-1、STAT-1磷酸化情况;激光共聚焦显微镜观察STAT-1核转位情况。结果:七叶皂苷钠可浓度依赖性抑制胃癌细胞增殖,使胃癌细胞形态及细胞核形态呈现凋亡变化,显著升高癌细胞凋亡率,并下调JAK-1、STAT-1信号蛋白磷酸化及抑制STAT-1的核转位。结论:七叶皂苷钠能抑制BGC-823细胞及AGS细胞增殖并诱导其凋亡,该过程可能是通过抑制JAK-1/STAT-1信号级联来实现的。

Objective： To investigate the mechanism of sodium aescinate on the apoptosis of gastric cancer BGC-823 cells and AGS cells. Methods： CCK-8 assay was used to detect the cell viability of cancer cells; cells morphological changes were observed by inverted microscope; changes in nuclear morphology were observed by fluorescence inverted microscope after DAPI staining; cells apoptosis rate was detected by flow cytometry; Western bloting was used to detected the phosphorylation of JAK-1 and STAT-1; laser confocal microscopy was used to observed the nuclear translocation of STAT-1. Results： Sodium aescinate inhibited cells proliferation in a concentration dependent manner,and changed the cells morphology and nuclear morphology,significantly up-regulated the apoptosis rate of gastric cancer BGC-823 cells and AGS cells,and down-regulated the phosphorylation of JAK-1,STAT-1 protein,inhibited the nuclear translocation of STAT-1. Conclusion： Sodium aescinate inhibit the proliferation and induce apoptosis in the gastric cancer BGC-823 cells and AGS cells,and this process can be implemented by inhibiting the JAK-1 / STAT-1 signaling pathway.