益气除痰方治疗脾虚型肺癌作用机制探讨

Study on the Mechanism of Yiqi Chutan Recipe in the Treatment of Lung Cancer with Splenasthenic Syndrome

目的:从内质网应激(ERS)角度探讨益气除痰方治疗脾虚型肺癌的作用机制。方法:将60只成功建模的脾虚Lewis肺癌小鼠随机分为模型组,顺铂注射液(1 mg/kg)阳性对照组及益气除痰方低(2 g/kg)、中(4 g/kg)、高(8 g/kg)剂量组,联合用药组(益气除痰方4 g/kg+顺铂注射液1 mg/kg),每组10只。给药14 d后处死小鼠,检测肿瘤体积和质量;HE染色观察肿瘤组织病理学改变;免疫组织化学法、Western blot法检测肿瘤组织中CHOP、DR5蛋白表达。结果:与模型组比较,除益气除痰方低剂量组外各给药组肿瘤体积、肿瘤质量显著降低(P<0.01),各给药组肿瘤组织中肿瘤细胞大量坏死,内质网应激蛋白CHOP、DR5表达显著升高(P<0.01),其中以联合用药组作用最佳。结论:益气除痰方和化疗对肺癌具有不同程度的抑制作用,联合用药效果更好,其作用机制可能与上调内质网应激蛋白CHOP、DR5表达介导细胞凋亡有关。

Objective： To investigate the mechanism of Yiqi Chutan recipe（ YCR） in the treatment of lung cancer with splenasthenic syndrome from the perspective of the endoplasmic reticulum stress（ ERS）. Methods： 60 mice were successfully modeled as Lewis lung cancer mice with spleen deficiency,which were randomly divided into model group（ normal saline）,cisplatin group（ 1 mg / kg）,low dose（ 2 g/kg） YCR group,middle dose（ 4 g/kg） YCR group,high dose（ 8 g/kg） YCR group and the combined medication group（ YCR 4 g/kg ＋ cisplatin 1 mg / kg）,with 10 mice in each group. The mice were killed after 14 days,the size and weight of tumor in mice were measured,morphological changes of tumor were observed by HE staining method,the expression of CHOP and DR5 protein in tumor tissue was detected by immunohistochemical method and Western blot method. Results： Compared with model group,the volume and weight of tumor in cisplatin group,middle dose YCR group,high dose YCR group and combined medication group were significantly decreased（ P〈0. 01）,and the tumor cells in transplanted tumor were large necrosis and the protein expression of CHOP and DR5 were increased significantly（ P〈0. 01）. The effect in treatment of lung cancer with splenasthenic syndrone in combined medication group was higher（ P〈0. 05 or P〈0. 01）. Conclusion： Yiqi Chutan recipe has different degrees of inhibition on lung cancer,the combined use of cisplatin can obviously improve the curative effect. The mechanism may be related to up-regulating the expression of CHOP and DR5 in endoplasmic reticulum stress-related proteins.