当归挥发油对高血脂小鼠动脉粥样硬化的保护作用

Protective Effects of Angelica sinensis Volatile Oil on Atherosclerosis in Hyperlipidemia Mice

目的:观察当归挥发油对高血脂小鼠动脉粥样硬化的保护作用。方法:60只昆明小鼠随机分为正常对照组、模型组、氟伐他汀阳性对照组及当归挥发油高、中、低剂量组,正常对照组喂食普通饲料,其他各组喂食高脂饲料,并每天灌胃维生素D3(100万IU/kg),连续42 d。造模第14 d,氟伐他汀组小鼠灌胃氟伐他汀(6.7 mg/kg),当归挥发油高、中、低剂量组分别灌胃当归挥发油(40、20、10 mg/kg),正常对照组和模型组灌胃等体积生理盐水,连续28 d。观察各组小鼠活动状态,称量体质量。末次给药1 h后用全自动生化仪检测血清TC、TG、HDL-C、LDL-C水平,计算动脉粥样硬化指数(AI1,AI2)和冠心指数(R-CHR)。处死小鼠,称量心、肝质量,计算脏器指数;心脏、肝脏和腹主动脉组织经甲醛固定,石蜡包埋,切片,HE染色,光镜下观察组织病理学改变。结果:与正常对照组比较,模型组小鼠体质量增长显著下降(P<0.01),心脏、肝脏指数显著升高(P<0.05),血清TC、TG、LDL-C、HDL-C水平及AI1、AI2和R-CHR均显著升高(P<0.01);与模型组比较,当归挥发油高、中剂量组小鼠体质量增长显著升高,心、肝指数均显著降低(P<0.05),当归挥发油低剂量组血清TC、TG、LDL-C水平显著降低(P<0.05或P<0.01),当归挥发油各剂量组AI1和R-CHR均显著降低(P<0.05或P<0.01),当归挥发油高剂量组AI2显著降低(P<0.05)。组织病理检测显示,当归挥发油能减轻肝细胞脂肪变性、胸主动脉内膜损伤及心肌纤维化,对动脉粥样硬化斑块形成具有抑制作用。结论:当归挥发油对高血脂小鼠动脉粥样硬化具有一定的保护作用。

Objective： To study the protective effects of Angelica sinensis volatile oil on atherosclerosis in hyperlipidemia mice. Methods： 60 mice were randomly divided into normal control group,model group,fluvastatin group,and high-,medium- and lowdose group of Angelica sinensis volatile oil. Normal control group were fed with normal diet,the other groups were fed with high fat diet,and treated orally Vitamin D3（ 100 million IU/kg） daily for 42 d. In 14 th day after modeling,fluvastatin group were orally administrated fluvastatin（ 6. 7 mg / kg）,and high-,medium- and low- dose of Angelica sinensis volatile oil groups were orally administrated Angelica sinensis volatile oil（ 40,20,10 mg / kg） for 28 d,and the normal control group and model group were administrated equal volume normal saline. Activity state,body weight and the levels of TC,TG,HDL-C and LDL-C in serum were measured,atherosclerosis indexes（ AI1,AI2）,coronary heart index（ R-CHR） were calculated. After the mice were killed,the heart,liver and abdominal aortas were taken. The mass of the heart and liver were measured,and the organ indexes were calculated; the tissue were fixed by formalin,embedded in paraffin,sliced,HE stained,and the histopathology changes were observed by microscope. Results： Compared with normal control group,the body weight of mice in the model group were decreased（ P〈0. 01）,and the heart,liver indexes were significantly increased（ P〈0. 05）,the levels TC,TG,LDL-C and HDL-C in serum and AI1,AI2 and R-CHR were significantly increased after modeling 42 d（ P〈0. 01）. Compared with the model group,the mice body weight were significantly increased,and the heart,liver index were significantly decreased（ P〈0. 05） in the high-,middle- dose group of Angelica sinensis volatile oil groups; TC,TG and LDL-C levels were significantly decreased in low- dose group（ P〈0. 05 or P〈0. 01）; AI1 and R-CHR were significantly decreased（ P〈0. 05 or P〈0. 01） in all Angelica sinenisis volatile oil groups,but AI2 in the high dose group of Angelica sinensis volatile oil was significantly decreased（ P〈0. 05）. Histopathology results showed that Angelica sinensis volatile oil could relieve the fatty degeneration of hepatic cells and the injury of thoracic aortic intimae,and myocardial fibrosis,which could inhibit the formation of atherosclerotic plaque. Conclusion： The certain protective effects of Angelica sinensis volatile oil are determinated on atherosclerosis in hyperlipidemia mice.