分子标记和辐射技术在小麦-长穗偃麦草抗赤霉病易位系创建中的应用

Application of Molecular Markers and Radiation in the Establishment of FHB Resistance Wheat-Thinopyrum elongatum Translocation Lines

为了打破小麦抗赤霉病育种种质资源狭窄的局限,丰富抗赤霉病遗传材料,本研究通过对中国春-长穗偃麦草二体异代换系DS7E/7A与扬麦16杂交后代进行辐射诱变,以产生小麦-长穗偃麦草易位系选择群体,并结合赤霉病抗性鉴定和长穗偃麦草染色体特异分子标记辅助选择,最终获得了5个小麦-长穗偃麦草抗赤霉病易位单株,创建了一条有效获得小麦-长穗偃麦草抗赤霉病易位系的技术路线。小麦-长穗偃麦草抗赤霉病易位系的成功创建不仅为小麦抗赤霉病育种提供了重要的中间材料,也将为小麦近缘种的抗赤霉病研究和利用提供理论与实践参考。

As one of the important wild relatives of wheat, some studies have showed that the 1E and 7E chromosomes of Thinopyrum elongatum have FHB resistance genes, which has been considered to be one important germplasm resource for wheat genetic improvement. In order to break the narrow limitation of germplasm resources for wheat＇ s FHB resistance breeding and enrich its genetic stocks, Chinese Spring-Thinopyrum elongatum disomic substitution line, DS7E/7A, was used to transfer the FHB resistance genes into common wheat. The hybrid offspring of DS7E/7A and Yangmai 16 were irradiated to produce wheat-Th, elongatum translocation lines. Then, they were identified and selected by FHB resistance identification and chromosome specific molecular markers of Th. elongatum. Finally, 5 FHB resistance wheat-Th, elongatum translocation lines were obtained, and one effective technical solution was accurately and effectively established. The success of this study not only provides important intermediate materials for wheat＇ s FHB resistance breeding, but also provides important theoretical and practical basis for research and utilization of wheat＇ s wild relatives.