马齿苋醇提物中羟基二氢博伏内酯的分离及结构鉴定

Isolation and Identification of Hydroxydihydrobovolide in Portulaca oleracea L.

目的:分离马齿苋(Portulaca oleracea L.)中乙醇提取物中的化学成分。方法:依次采用50%乙醇回流提取、大孔吸附树脂、乙酸乙酯萃取、硅胶柱层析、ODS中压反相柱和Sephadex LH-20对马齿苋中的化学成分进行提取、分离、纯化,最后采用超高效液相色谱进行制备和结构鉴定。结果:1H-NMR、13C-NMR、DEPT、HR-ESI-TOF-MS及二维HSQC、HMBC、NOESY、COSY的核磁共振图谱解析,得到化合物命名为5-羟基-3,4-二甲基-5-戊烷基-2(5H)-呋喃酮,即羟基二氢博伏内酯。结论:羟基二氢博伏内酯为在马齿苋中首次分离得到。

Objective：The purpose is to study the compound in the Portulaca oleracea L. Methods：Separate the compound successively through macroretieular resin, ethyl acetate extraction, polyamide resin, silica gel column and ODS, purified with Sephadex LH - 20, and then use the ultra - high performance liquid chromatography （ UHPLC ） and nuclear magnetic resonance （ NMR ） methods. Results ： A compound was obtained at last via analyzing the graphs of ^1H - NMR, ^13 C - NMR, HR - ESI - TOF - MS and HSQC, HMBC, NOESY, COSY. Conclusion：Hydroxydihydrobovolide （HDB） was for the first time isolated from the leaves of Portulaca oleracea L..