阿魏菇菌丝体多糖的分离纯化研究

Separation and Purification of Pleurotus Mycelium Polysaccharide

以液体发酵获得的阿魏菇菌丝体为材料,开展了阿魏菇菌丝体多糖分离纯化及纯度鉴定研究。结果表明,料液以质量体积比1∶15(g/m L)、提取温度100℃、提取时间3 h、提取次数3次,热水浸提,用孔径38μm的尼龙布过滤,加入适量20%三氯乙酸去蛋白,5倍体积于多糖浓缩液的无水乙醇醇析10 h,10%的H2O2脱色8 h和DEAE-52柱层析纯化处理得到的阿魏菇菌丝体多糖,经吸收光谱扫描不含蛋白、核酸及色素残留,经红外光谱扫描初步判定其为糖类物质,且含有β-糖苷键。

Taking Pleurotus mycelium obtained through liquid fermentation as materials,the separation,purification and identification of Pleurotus mycelium polysaccharide were also made. The results showed that the fresh mycelium was extracted with deionized water [material-water ratio：1 ∶ 15（g/m L）,extraction temperature：100 ℃,extraction time：3 h and extraction degree：3 times],then the supernatant was separated from insoluble residue with nylon cloth（pore diameter 38 μm）. The extracts were then removed protein by the addition of suitable 20 % trichloroacetic acid,precipitated by the addition of anhydrous ethanol（ethanol-polysaccharide concentration ratio 5 ∶ 1,precipitation time 10 h）,and the precipitates were collected by centrifugation,then solubilized in deionized water and bleached with 10 % H_2O_2 to get the crude polysaccharides. And then the crude polysaccharides were purified with DEAE-52 column chromatography to get the mycelium polysaccharides. The mycelium polysaccharides did not contain proteins,nucleic acids and pigment residue by absorption spectrum scanning,were determined initially as carbohydrate and contained a β-glycosidic bond by IR scan.