地椒挥发油提取工艺及GC-MS成分分析

Extraciton Process of Volatile Oil from Thymus quinquecostatus Celak and Analysis of Chemical Components by GC-MS

确定地椒挥发油的最佳提取工艺并对其进行成分分析。通过单因素试验,确定了影响挥发油提取的4个因素;选用L9（3^4）正交试验,以挥发油得率为指标,对影响挥发油收率的提取时间（A）、浸泡时间（B）、粉碎度（C）、加水量（D）4个因素进行提取工艺条件优选,并按最佳提取工艺提取的挥发油进行气相色谱-质谱（GC-MS）分析。结果发现,地椒挥发油的最佳提取工艺为药材粗粉加入10倍量的水、浸泡2 h、提取4 h,并鉴定出了63种挥发性化学成分。

To study the optimum extraction technology and the chemical constituents of volatile oil from Thymus quinquecostatus Celak. Single factor test method was used for studying the effects of four extracting factors.With the yield of volatile oil as index,orthogonal experimental design L9（3^4）was used for studying the effects of four extracting factors these were extracting time（A）,soaking time（B）,crushing degree（C）and the amount of water（D）. Constituents of volatile oil extracted were identified by the application of GC-MS techniques. The optimal condition for the extraction of volatile oil from Thymus quinquecostatus Celak was：powdery medicinal materials,ten times water,soaked for 2 hours and extracting time at four hours. 63 kinds the chemical constituent of volatiles were identified.