不同溶剂提取牦牛乳硬质干酪脂肪酸的GC-MS分析

GC-MS analysis of fatty acids from yak milk hard cheese by different solvents

【目的】合理评价牦牛乳硬质干酪的品质及营养价值.【方法】以甘肃天祝牧区新鲜牦牛乳制作的硬质干酪为原料,分别用石油醚和氯仿作为溶剂提取脂肪酸,通过气相色谱-质谱联用仪(GC-MS)对脂肪酸的组成及含量进行测定和分析.【结果】石油醚溶剂提取物中共检出46种脂肪酸成分,氯仿溶剂提取物中共检出36种;在两种溶剂提取物中,均以棕榈酸、(E)-9-十八烯酸、硬脂酸、肉豆蔻酸和11-十八烯酸5种脂肪酸的含量较高.【结论】石油醚作为溶剂提取的脂肪酸种类多,含量高,提取效果更好,是研究与分析牦牛乳硬质干酪脂肪酸成分与其含量较理想的提取溶剂.

[Objective] The test aimed at providing scientific basis ior evaluating the value of yak milk-hard cheese. [Method] Taking hard cheese from yak milk in grazing pastures of Tianzhu county, Gansu province as raw material, the fatty acids from hard cheese was extracted by petroleum ether and chloroform, respectively and the fatty composition was detected by GC-MS. [Result] The result showed that the fatty acids extracted from hard cheese contained 46 fatty acid components by petroleum ether and 36 by chloroform. Under the two solvent extraction conditions, the content of Palmitic acid, （E） -9-octadecenoic acid, stearic acid, myristic acid and l l-octadecenoic acid was higher. [Conclusion] The petroleum ether extracted more kinds and higher content of fatty acids, it was the ideal extraction solvent to analyze fatty acid composition and content of yak milk hard cheese.