人参辅酶Q_(10)防晒霜对紫外线损伤小鼠的保护作用

Protective effect of ginseng co-enzyme Q_(10) suncream on the ultraviolet radiation-induced skin damage in mice

目的研究人参辅酶Q_(10)防晒霜对紫外线损伤小鼠的保护作用。方法 36只小鼠随机分组,皮肤脱毛后分别涂抹相应霜剂,正常对照组不做任何处理,其他组进行紫外线照射,连续8周后,小鼠眼球取血处死进行指标检测。结果与正常组小鼠比较,模型组小鼠皮肤粗糙有皱纹、且角质层脱落,T-SOD与GSH-Px活力均明显下降,白细胞数与MDA含量明显增高(P<0.05);与模型组比较,人参辅酶Q_(10)防晒霜组小鼠皮肤光滑、角质层没有脱落,T-SOD活力、红细胞与血红蛋白含量上升,白细胞数量、MDA含量与明显下降(P<0.05),阳性对照组小鼠皮肤光滑、角质层没有脱落,GSH-Px活力上升,白细胞数量、MDA含量下降(P<0.05)。结论人参辅酶Q_(10)防晒霜具有抗紫外线损伤的作用,其预防效果与甲氧基肉桂酸辛酯辅酶Q_(10)霜剂相当。

Objective To explore the effect of ginseng co-enzyme Q10 suncream on the skin damage caused by ultraviolet（ UV） radiation in mice. Methods 36 mice were randomly assigned to four groups. The mice were shaved on the back and the left untreated side was taken as control group,or was treated with UV as model group. Before treated with UV,the mice were painted with suncream containing ginseng co-enzyme Q10,or octyl methoxycinnamate as positive controls. The mice were treated for 8 weeks. At the end of the experiment,blood samples of all mice were collected from the eyes,then subjected to cell counting or biochemical measurements,and skin samples were cut for pathological examination. Results Compared with the control group,there was a significant increase in white blood cell counts（ P〈0. 05）and MDA content（ P〈0. 05）,and declined serum levels of SOD（ P〈0. 05） and GSH-Px（ P〈0. 05） in the model group,and the skin was rough and wrinkled with stratum corneum exfoliation. Compared with the model group,the mice of ginseng co-enzyme Q10 suncream group had significantly lower white blood cell count（ P〈0. 05） and MDA content（ P〈0. 05）,and increased serum levels of T-SOD（ P〈0. 05） and red blood cell counts（ P〈0. 05）. The skin had no roughness and wrinkles and without stratum corneum exfoliation. Compared with the model group,the positive control group showed significantly decreased white blood cell count（ P〈0. 05） and MDA content（ P〈0. 05）,and increased serum levels of GSH-Px（ P〈0. 05）. The skin had no roughness and wrinkles and no stratum corneum exfoliation. However,there was no significant difference between the ginseng co-enzyme Q10 suncream group and positive control group. Conclusions Ginseng co-enzyme Q10 suncream shows satisfactory preventive effects on the UV radiation-induced skin damage in mice,similar to the preventive effects of the octyl methoxycinnamate-containing sunsream.