西藏小型猪IGF-1基因在不同生长发育阶段和不同组织器官中的差异表达

Differential IGF-1 gene expression in various tissues at different developmental stages in Tibet minipigs

目的测定西藏小型猪的IGF-1基因在不同年龄阶段、不同组织中的表达分布情况,为未来的研究提供基础数据。方法采用实时荧光定量PCR技术,以GAPDH基因为内参照,检测了IGF-1基因在西藏小型猪不同组织和不同生长阶段间的表达变化。结果 (1)IGF-1基因表达的时序性研究表明在0、0.25、0.5、1、2岁时肌肉组织的表达量最低,在0、0.25、0.5岁时皮肤组织中表达量最高,在0.1、2、3岁时肝组织的表达量最高。(2)IGF-1基因在在7个不同组织中均有表达,0.25岁时其在表达丰度由高到低依次为:皮肤、肺、肝、肾、脾、心脏和肌肉。且在皮肤中的表达量最高且极显著高于其他各组织(P<0.01)。同时在不同年龄阶段的表达中,IGF-1基因0.25岁时在各组织中的表达均达到峰值。结论西藏小型猪的IGF-1基因的表达呈现出明显的时空特异性。

Objective To determine the expression and distribution of IGF-1 gene in different tissues of Tibet minipigs at different ages. Methods The changes of IGF-1 gene expression in different tissues and different growth stages of Tibet minipigs were detected by quantitative real-time fluorescence PCR technology and GAPDH gene was used as the reference. Results（ 1） The study of sequential expression of IGF-1 gene showed that the lowest expression of IGF-1 gene in muscle tissue was at 0,0. 25,0. 5,1,and 2 years of age,whereas the highest expression of IGF-1 gene in skin tissue was at 0,0. 25,0. 5 years of age and the highest expression of IGF-1 gene in liver tissue was at 0. 1,2,3 years of age.（ 2） IGF-1 gene was expressed in all the 7 examined tissues,and its expression level was in the following decreasing order：skin,lung,liver,kidney,spleen,heart and muscle at 0. 25 years of age. The expression of IGF-1 gene in the skin was significantly higher than in other organ tissues（ P〈0. 01）. Conclusions The expression of IGF-1 gene of Tibet minipigs showed obvious temporal and spatial specificity.