经皮肤暴露四溴双酚A对Wistar雄性大鼠的毒性效应

Toxicity Effects of Tetrabromobisphenol A in Male Wistar Rats Following Dermal Exposure

尘/土以及含阻燃剂产品的皮肤接触是四溴双酚A(TBBPA)重要的人体暴露途径。为研究TBBPA经皮肤亚慢性暴露毒性效应,选择无特定病原菌级别(SPF级)Wistar雄性大鼠作为受试生物,分别设空白对照组(K)、溶剂对照组(Z),以及20mg·kg^(-1)(A)、60 mg·kg^(-1)(B)、200 mg·kg^(-1)(C)、600 mg·kg^(-1)(D)共4个不同剂量的TBBPA暴露组,采用皮肤接触的方式连续暴露90 d。暴露期间观察大鼠状态并称重,于第91天解剖大鼠,分离主要脏器称重计算脏器系数,并对暴露皮肤进行组织病理学检查。研究发现,经皮肤暴露TBBPA后,90 d暴露期间不同实验组Wistar大鼠在表观形态、行动、进食方面无差异,TBBPA暴露导致大鼠体重略微降低,但各处理组间大鼠体重无显著差异;90 d暴露后不同剂量组间大鼠的器官脏器系数没有显著的剂量-效应关系,不同剂量组大鼠皮肤暴露区域均有一定程度的炎症细胞浸润及部分组织胶原间隙增宽。研究结果表明,TBBP A 90 d亚慢性皮肤暴露对Wistar大鼠无明显毒性效应。

Dermal contact for dust and consumer products has been identified as the major exposure pathways to tetrabromobisphenol A（ TBBP A）. In order to reveal the subchronic toxic effects,the male Wistar rats were exposed for 90 days to TBBP A dosage varying at 20,60,200 and 600 mg·kg^-1body weight,and the similar experiments were also conducted in blank control group and solvent control group. The results showed that within the 90-d dermal contact with TBBP A,the significant difference concerning animal growth,behaviour and feeding among allgroups was not clearly observed（ P〉0. 05）,including the organ coefficients of thymus,heart,lung,liver,kidneys,spleen,ovary,and brain between treatment groups and the control or vehicle group,although a slight change in body weight and a certain degree of inflammatory cell infiltration and collagen gap widened was observed. It is indicated that the subchronic dermal exposure to TBBP A could not cause significantly toxicological effects.