申克氏孢子丝菌DRK1启动子检测载体的构建

孢子丝菌病的病原体为申克氏孢子丝菌,该病发病率高,可引起动物皮肤、皮下组织的慢性感染,导致化脓、溃烂及渗出等症状。深入研究孢子丝菌形态转换过程有助于揭示孢子丝菌病的发病机制。笔者通过PCR扩增申克氏孢子丝菌DRK1的终止子序列,将其克隆到载体pEGFP-N1下游,又通过PCR扩增申克氏孢子丝菌DRK1的启动子序列,将其克隆到载体pEGFP-N1上游,构建检测孢子丝菌DRK1启动子的载体,为导入PCB309菌检测启动子奠定基础。

The pathogen of sporotrichosis for sporothrix schenckii, high rates of the disease, can cause animals can cause chronic infection of the skin, subcutaneous tissue, leading to fester, the symptom such as canker and leak.Further study of spore silk morphology transformation process helps to reveal the pathogenesis of sporotrichosis. This experiment by PCR amplification ShenKeShi spore silk DRK1 termination sequence, the cloning pEGFP - N1 downstream to the carrier, and by PCR amplification ShenKeShi spore silk DRK1 promoter sequence, to clone it to carrier pEGFP - N1 upstream, build testing spore silk DRK1 promoter of carrier, promoter to lay the foundation for import PCB309 bacteria detection.