不同产地神曲甲醇提取物对巨噬细胞及小肠上皮细胞活性的影响

Effects of Methanol Extracts of Different Habitat Massa Medicata Fermentata( MMF) on the Activity of Macrophages and Small Intestinal Epithelial Cells

目的比较不同产地神曲对小鼠RAW264.7单核-吞噬细胞及大鼠小肠上皮细胞株IEC-6细胞活性的影响。方法不同产地神曲样品甲醇提取,氮吹仪下挥干溶剂,加入空白培养基配成1g/ml母液,无菌条件下加DMEM空白培养基使母液稀释成浓度分别为800、400、200、100、0μg/ml,对大鼠小肠上皮细胞株IEC-6细胞及小鼠RAW264.7单核-吞噬细胞进行给药培养,检测二者增殖率、巨噬细胞吞噬能力及其所分泌TNF-α的水平。结果不同神曲样品在低给药浓度(100、200μg/ml)时均有促进IEC-6细胞及RAW264.7细胞增殖的作用,尤其以中国药材公司采集的神曲炒品(Z炒神曲)效果较显著(P<0.05)。对于巨噬细胞吞噬能力及其所分泌的TNF-α水平,各组间虽差异无统计学意义,但仍以Z炒神曲在较低浓度时优于其他组别。结论不同产地神曲对小肠上皮IEC-6细胞及巨噬细胞RAW264.7活性有促进作用,以从中国中药公司采集的Z炒品效果较优。

Objective To compare the effect of different habitat Massa Medicata Fermentata on the activity of the mouse mononuclear macrophages RAW264.7 and rat small intestinal epithelial IEC-6 cells. Methods Different MMF samples wera extracted with methanol drying solvent with nitrogen blowing instrument,1g/ml initial liquid was made with blank culture medium in a aseptic condition,the initial liquid was diluted into 800,400,200,100,0μg/ml with DMEM blank culture medium to culture mouse mononuclear macrophages RAW264.7 and rat small intestinal epithelial IEC-6 cells or determine the both appreciation rate,macrophage phagocytic ability and the levels of TNF-α. Results Different MMF samples had the effect of promoting the proliferation of IEC-6 cells and RAW264.7 cells at low concentration（100,200μg/ml,which especially remarkable was fried product collected from Chinese traditional Chinese medicine company（Z fried MMF）（P〈0.05）.Although there was no significant difference between the groups for the phagocytic ability of macrophages and its levels of TNF-α, Z fried product was better than other groups at low concentrations. Conclusion Different MMF samples have a promoting effect on the activity of intestinal epithelial IEC-6 cells and macrophage RAW264.7 cells.Z fried product collected from Chinese traditional Chinese medicine company is better.