摘要
目的探讨微小RNA-30b(miRNA-30b)在非小细胞肺癌(NSCLC)中的表达情况,并进一步分析miR-30b与NSCLC患者预后的相关性。方法收集41例NSCLC患者的肿瘤组织及其正常组织(距离肿瘤组织>5cm),采用real-time PCR法检测miR-30b的表达情况,并对预后进行分析。结果肿瘤组织miR-30b的表达水平显著低于正常组织(P<0.01);miR-30b的表达水平与临床分期、淋巴结转移及EGFR基因突变显著相关(P<0.05)。Kaplan-Meier分析显示肿瘤组织中miR-30b高表达组的生存时间明显高于低表达组(P<0.05)。结论 MiR-30b在NSCLC中的表达是下调的,可作为NSCLC预后的一个新的潜在标志物。
Objective To investigate the expression of microRNA-30b（miRNA-30b） in patients of non-small cell lung cancer（NSCLC） and to analyse the association of its expression and prognosis. Methods The expression of miR-30b in 41 tumor tissues and paired normal tissues of NSCLC were detected by RT-PCR.The relationship between the expression levels of miR-30b and the prognosis of patients was analysed. Results The expression level of miR-30b was up-regulated in tumor tissues while compared with paired normal tissues（P〈0.05）.The expression of miR-30b was associated with TNM stages,lymphatic metastasis and the genetic mutation of EGFR（P〈0.05）.Kaplan-Meier analyse reveled that the overall survival of high-expression group was longer than the low-expression group（P〈0.05）. Conclusion The expression of miR-30b in NSCLC is down-regulated,and it is maybe a novle evaluation of predicting the prognosis of NSCLC.
出处
《医学研究杂志》
2017年第2期63-65,82,共4页
Journal of Medical Research
基金
浙江省医药卫生科技计划项目(2015KYA239)
