大蒜素抑制人子宫内膜癌Ishikawa细胞生长的实验研究

Experimental Study of Allitridi Inhibitting the Growth of Endometrial Lshikawa Cancer Cell

目的研究大蒜素抑制人子宫内膜癌Ishikawa细胞生长的作用,并探讨其可能的作用机制。方法以不同浓度大蒜素(12.5、25、50μg/ml)和顺铂(40μg/ml)分别干预对数生长期Ishikawa细胞,采用MTT法检测Ishikawa细胞增殖抑制;通过流式细胞术检测细胞周期和细胞凋亡状况,采用Western blot法检测Ishikawa细胞中caspase-3蛋白表达,RT-PCR法检测Ishikawa细胞中Bax mRNA、bcl-2 mRNA表达并计算Bax/bcl-2表达比值。结果大蒜素能够显著提高子宫内膜癌Ishikawa细胞增殖抑制率、阻滞Ishikawa细胞周期于G_2/M期,提高细胞凋亡率、上调caspase-3蛋白和Bax mRNA表达、下调bcl-2 mRNA表达、提高Bax/bcl-2表达比值,且上述作用均具有一定的剂量依赖性。结论大蒜素对子宫内膜癌Ishikawa细胞增殖具有抑制作用以及促其凋亡的作用,从而表现出其抑制Ishikawa细胞生长的作用;作用机制可能与大蒜素能够改变Ishikawa细胞周期以及调节凋亡相关基因、蛋白表达有关。

Objective To investigate the inhibiting effects of Allitridi on the growth of endometrial Ishikawa cancer cell. Methods The endometrial Ishikawa cancer cells in logarithmic period were treated with different concentration of Allitridi（12.5, 25, 50μg/ml） and Cisplatin（40μg/ml）. The cellular growth inhibition rate was calculated by MTT, cell cycle and apoptosis rate were analyzed by flow cytometry. The expression of caspase-3 protein expression was detected by Western blotting, the expression of Bax mRNA and bcl-2 mRNA were detected by RT-PCR, and the ratio of Bax/bcl-2 was calculated. Results Allitridi had the effects of increasing the cellular growth inhibition rate, arresting the cell cycle at G2/M phase, increasing the apoptosis rate, up-regulating the expression of caspase-3 protein and Bax mRNA, down regulating the expression of bcl-2 mRNA, increasing the ratio of Bax/bcl-2, which were dose-depented. Conclusions Allitridi had inhibitive effecfs on the proliferation and apoptosis of endometrial Ishikawa cancer cell, which perhaps related to its effects of changing the Ishikawa cell cycle and regulating the expression of apoptosis-related genes and protein expression.