藏药榜嘎生物碱成分分离及质量标准研究

Research on Alkaloid Constituents and Quality Standards of Bangga

目的:分离、鉴定榜嘎中生物碱成分,并建立其质量标准。方法:采用醇提配合酸碱萃取法得到总生物碱,经硅胶柱层析法分离得到单体生物碱并鉴定其结构;采用HPLC对不同产地榜嘎进行共有成分分析,以共有成分作为参照,结合薄层色谱法对榜嘎药材进行鉴别;按照《中国药典》2010年版(一部)附录方法对榜嘎药材水分、总灰分、酸不溶性灰分进行测定;采用HPLC法测定共有成分含量。结果:分离得到大麦芽碱、异叶乌头碱、塔拉乌头胺3个单体化合物;大麦芽碱为榜嘎药材共有成分,建立了以其为指标成分的薄层鉴别方法及含量测定方法;12批榜嘎药材中大麦芽碱平均含量为0.026%;拟定榜嘎药材水分不得超过9.5%,总灰分不得超过16.5%,酸不溶性灰分不得超过2.9%。结论:以大麦芽碱为对照的定性、定量方法操作简单、稳定性和重复性良好,可用于藏药榜嘎的质量控制。

Objective：To study the isolation and identification of the alkaloids and to establish quality standards of Bangga. Methods：The total alkaloids were obtained by alcohol extraction and acid-base partition. The alkaloids were isolated by silica gel column chromatography, and their structures were identified by UV, IR, NMR, and MS. HPLC was carried on the qualitative analysis of the common ingredient from the different origins to provide the standard for establishing quality standards of Bangga. According to Chinese Pharmacopoeia （2010 edition）, the methods of a thin layer chromatography （TLC） and HPLC, content of moisture, total ash, and acid-insoluble ash were established to control quality of Bangga. Results： Heteratisine, hordenine, and talatizamine were obtained and identified, and the common alkaloid was hordenine by HPLC analysis. A thin layer chromatography （TLC） and HPLC were established with hordenine as index ingredient for identification and determination of Bangga. The average content of hordenine is 0. 026%. The moisture, total ash, and acid-insoluble ash of Bangga were suggested that should not be more than 9.5%, 16.5%, and 2.9 %, respectively. Conclusion ： The method, established with hordenine as index ingredient for identification and determination of Bangga was simple to operate with good stability and reproducibility, which can be used for quality control of the Tibetan med icine Bangga.