软性蝶骨电极在幼鼠长程脑电监测中的应用研究

Flexible sphenoidal electrodes in long-term electroencephalogram monitoring in infant rats

目的探讨软性蝶骨电极在幼鼠长程脑电监测中的应用。方法健康清洁级4周龄Wistar大鼠30只按随机数字表法分为蝶骨电极组（n=15）与硬性电极组（n=15）。应用腹腔注射氯化锂一匹鲁卡品方法诱发幼鼠癫痫持续状态．2周后2组幼鼠分别手术置入软性蝶骨电极及自制硬性电极，并进行连续2周视频脑电监测，记录幼鼠癫痫发作次数及电极故障脱落发生率。结果蝶骨电极组电极置人过程顺利，电极固定牢固；硬性电极组因幼鼠颅骨面积有限和置入物重量因素，电极常常固定不稳。蝶骨电极组幼鼠监测期间平均癫痫发作（37．3±6．21次，硬性电极组幼鼠监测期间平均癫痫发作（36．9±5．8）次，比较差异无统计学意义（t=0．180．P=0．430）。蝶骨电极组脑电记录信号清晰稳定，信噪比高，无电极故障脱落；硬性电极组脑电记录信号欠佳，信噪比低，8只（53.3％）幼鼠在监测过程中出现电极脱落；2组间电极故障脱落发生率比较差异有统计学意义（χ2=10．900，P=0．001）。结论软性蝶骨电极用于大鼠（尤其是幼鼠）进行长程脑电监测，可行性好，电极故障脱落发生率低，脑电记录信号清晰稳定，值得推荐。

Objective To discuss the application of flexible sphenoidal electrodes in the long-term electroencephalogram （EEG） monitoring in infant rats. Methods A total of 30 male Wistar rats were randomly divided into two groups： sphenoidal-electrode group and traditional-electrode group （n=15）. Status epilepticus was induced in all animals by intraperitoneal injection of lithium and pilocarpine. Two weeks later, the rats of sphenoidal-electrode group were implanted with flexible sphenoidal electrodes and those of traditional-electrode group were implanted with home-made electrodes; long-term video-EEG monitoring was performed for two weeks. The quantity of seizures and rate of electrode falling were counted. Results In the sphenoidal-electrode group, all electrodes were successfully implanted and steadily fixed; there were average 37.3 ±6.2 times of seizure and none of electrodes fallen off during EEG recording; stable signal and high signal to noise ratio were noted. In the traditional-electrode group, both limited size and fragility of the immature skulls, out of bearing the weight of a solid implant that contained more electrodes, induced unstable implantation; there were average 36.9±5.8 times of seizure and eight electrodes （53.3%） fallen off during EEG recording; poor signal and low signal to noise ratio were noted. The average times of seizure showed no significant difference between the two groups （t=0.180, P=0.430）. The electrode falling rate in the sphenoidal electrode group was significantly lower than that in the traditional-electrode group （χ2=10.900, P=0.001）. Conclusion The flexible sphenoidal electrodes have low rate of electrode falling than traditional electrodes when processing EEG monitoring in infant rats, enjoying good feasibility and stable signal.